Structural basis for aminoglycoside inhibition of bacterial ribosome recycling.

Structural basis for aminoglycoside inhibition of bacterial ribosome recycling. Research Abstract Details 

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Structural basis for aminoglycoside inhibition of bacterial ribosome recycling. Abstract Text:

Maria A Borovinskaya,Raj D Pai,Wen Zhang,Barbara S Schuwirth,James M Holton,Go Hirokawa,Hideko Kaji,Akira Kaji,Jamie H Doudna Cate,

Aminoglycosides are widely used antibiotics that cause messenger RNA decoding errors, block mRNA and transfer RNA translocation, and inhibit ribosome recycling. Ribosome recycling follows the termination of protein synthesis and is aided by ribosome recycling factor (RRF) in bacteria. The molecular mechanism by which aminoglycosides inhibit ribosome recycling is unknown. Here we show in X-ray crystal structures of the Escherichia coli 70S ribosome that RRF binding causes RNA helix H69 of the large ribosomal subunit, which is crucial for subunit association, to swing away from the subunit interface. Aminoglycosides bind to H69 and completely restore the contacts between ribosomal subunits that are disrupted by RRF. These results provide a structural explanation for aminoglycoside inhibition of ribosome recycling.

Structural basis for aminoglycoside inhibition of bacterial ribosome recycling. Publishing Authors By Initials

MA Borovinskaya,RD Pai,W Zhang,BS Schuwirth,JM Holton,G Hirokawa,H Kaji,A Kaji,JH Cate,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: structure-activity relationship research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: structure-activity relationship research

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Structural basis for aminoglycoside inhibition of bacterial ribosome recycling. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Nature structural & molecular biology

VOLUME: 14

Page Numbers: 727-32

Journal Abbreviation: Nat. Struct. Mol. Biol.

ISSN: 1545-9993

DAY: 29

MONTH: 07

YEAR: 2007

Structural basis for aminoglycoside inhibition of bacterial ribosome recycling. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101186374

Structural basis for aminoglycoside inhibition of bacterial ribosome recycling. Keywords Mesh Terms:

KEYWORDS: Structure-Activity Relationship

MESH TERMS: chemistry

Chemical & Substance for Abstract: Structural basis for aminoglycoside inhibition of bacterial ribosome recycling. Information

Substance Name: Paromomycin

Registry Number: 7542-37-2

Grant and Affiliation Information for Structural basis for aminoglycoside inhibition of bacterial ribosome recycling.

AFFILIATION: Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.

Country: United States

AGENCY: United States NCRR

GRANT: RR-15301

ACRONYM: RR

MEDLINETA: Nat Struct Mol Biol

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