Structural and functional characterization of CC chemokine CCL14.

Structural and functional characterization of CC chemokine CCL14. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Structural and functional characterization of CC chemokine CCL14. Abstract Text:

Katherine Y Blain,Witek Kwiatkowski,Qinghai Zhao,David La Fleur,Chethana Naik,Tae-Wook Chun,Tatiana Tsareva,Palanisamy Kanakaraj,Michael W Laird,Rutul Shah,Lisa George,Indra Sanyal,Paul A Moore,Borries Demeler,Senyon Choe,

CC chemokine ligand 14, CCL14, is a human CC chemokine that is of recent interest because of its natural ability, upon proteolytic processing of the first eight NH2-terminal residues, to bind to and signal through the human immunodeficiency virus type-1 (HIV-1) co-receptor, CC chemokine receptor 5 (CCR5). We report X-ray crystallographic structures of both full-length CCL14 and signaling-active, truncated CCL14 [9-74] determined at 2.23 and 1.8 A, respectively. Although CCL14 and CCL14 [9-74] differ in their ability to bind CCR5 for biological signaling, we find that the NH2-terminal eight amino acids (residues 1 through 8) are completely disordered in CCL14 and both show the identical mode of the dimeric assembly characteristic of the CC type chemokine structures. However, analytical ultracentrifugation studies reveal that the CCL14 is stable as a dimer at a concentration as low as 100 nM, whereas CCL14 [9-74] is fully monomeric at the same concentration. By the same method, the equilibrium between monomers of CCL14 [9-74] and higher order oligomers is estimated to be of EC1,4 = 4.98 microM for monomer-tetramer conversion. The relative instability of CCL14 [9-74] oligomers as compared to CCL14 is also reflected in the Kd's that are estimated by the surface plasmon resonance method to be approximately 9.84 and 667 nM for CCL14 and CCL14 [9-74], respectively. This approximately 60-fold difference in stability at a physiologically relevant concentration can potentially account for their different signaling ability. Functional data from the activity assays by intracellular calcium flux and inhibition of CCR5-mediated HIV-1 entry show that only CCL14 [9-74] is fully active at these near-physiological concentrations where CCL14 [9-74] is monomeric and CCL14 is dimeric. These results together suggest that the ability of CCL14 [9-74] to monomerize can play a role for cellular activation.

Structural and functional characterization of CC chemokine CCL14. Publishing Authors By Initials

KY Blain,W Kwiatkowski,Q Zhao,D La Fleur,C Naik,TW Chun,T Tsareva,P Kanakaraj,MW Laird,R Shah,L George,I Sanyal,PA Moore,B Demeler,S Choe,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus internalization research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus internalization research

PUBMED ID PMID:

MEDLINE DATE:

Structural and functional characterization of CC chemokine CCL14. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Biochemistry

VOLUME: 46

Page Numbers: 10008-15

Journal Abbreviation: Biochemistry

ISSN: 0006-2960

DAY: 11

MONTH: 08

YEAR: 2007

Structural and functional characterization of CC chemokine CCL14. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370623

Structural and functional characterization of CC chemokine CCL14. Keywords Mesh Terms:

KEYWORDS: Virus Internalization

MESH TERMS: drug effects

Chemical & Substance for Abstract: Structural and functional characterization of CC chemokine CCL14. Information

Substance Name: Endopeptidases

Registry Number: EC 3.4.-

Grant and Affiliation Information for Structural and functional characterization of CC chemokine CCL14.

AFFILIATION: Structural Biology Laboratory, The Salk Institute, La Jolla, California 92037, USA.

Country: United States

AGENCY: United States PHS

GRANT: RRR022200

ACRONYM: HD

MEDLINETA: Biochemistry

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER: 2Q8T

Number Hits: 0

Structural and functional characterization of CC chemokine CCL14 Related Publications

• A matched controlled study to evaluate the efficacy of acupuncture for improving pregnancy rates following in vitro fertilization-embryo transfer.
• A survey of community-associated methicillin-resistant Staphylococcus aureus in Korea.
• BMP-3 and BMP-6 structures illuminate the nature of binding specificity with receptors.
• Change of biophysical properties of the skin caused by ultraviolet radiation-induced photodamage in Koreans.
• Clinical features and outcome of community-onset bloodstream infections caused by extended-spectrum beta-lactamase-producing Escherichia coli.
• Cloning and expression of human adenylate kinase 2 isozymes: differential expression of adenylate kinase 1 and 2 in human muscle tissues.
• Cocaine increases immunoglobulin heavy chain binding protein and caspase-12 expression in the rat dorsal striatum.
• Crystal structure of BMP-9 and functional interactions with pro-region and receptors.
• Detailed kinetics of immune responses to a new cell culture-derived smallpox vaccine in vaccinia-naïve adults.
• Diagnostic usefulness of a T-cell based assay for extrapulmonary tuberculosis.
• Dietary Korean mistletoe enhances cellular non-specific immune responses and survival of Japanese eel (Anguilla japonica).
• Evolution in the symbolic dimension: The devil is in the details.
• Human Follicular Dendritic Cells Interact with T Cells via Expression and Regulation of Cyclooxygenases and Prostaglandin E and I Synthases.
• In vitro ability of Staphylococcus aureus isolates from bacteraemic patients with and without metastatic complications to invade vascular endothelial cells.
• Meniscal gymnastics: common and uncommon locations of meniscal flip and flop.
• Modified Youngswick procedure for hallux limitus.
• NMR studies of interactions between C-terminal tail of Kir2.1 channel and PDZ1,2 domains of PSD95.
• Neural model of disinhibitory interactions in the modified Poggendorff illusion.
• Outcome of Vancomycin Treatment in Patients with Methicillin-Susceptible Staphylococcus aureus Bacteremia.
• Oxidation of corn oil during frying of soy-flour-added flour dough.
• Prediction of pathological stages before prostatectomy in prostate cancer patients: analysis of 12 systematic prostate needle biopsy specimens.
• Prediction of residual immunity to smallpox, by means of an intradermal skin test with inactivated vaccinia virus.
• Prenatal MRI of heteropagus twins.
• Protriptyline block of the human ether-à-go-go-related gene (HERG) K(+) channel.
• Sex preferences in Jeju pony foals (Equus caballus) for mutual grooming and play-fighting behaviors.
• Structural and functional characterization of CC chemokine CCL14.
• Structural systems identification of genetic regulatory networks.
• Synergistic activation of p70S6 kinase associated with stem cell factor in MO7e cells.
• Targeted inhibition of Pdp1epsilon abolishes the circadian behavior of Drosophila melanogaster.
• Treatment of hemoptysis in patients with cavitary aspergilloma of the lung: value of percutaneous instillation of amphotericin B.