Structural and Biophysical Characterization of Human myo-Inositol Oxygenase.

Structural and Biophysical Characterization of Human myo-Inositol Oxygenase. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Structural and Biophysical Characterization of Human myo-Inositol Oxygenase. Abstract Text:

Altered inositol metabolism is implicated in a number of diabetic complications. The first committed step in mammalian inositol catabolism is performed by myo-inositol oxygenase (MIOX), which catalyzes a unique four-electron dioxygen-dependent ring cleavage of myo-inositol to d-glucuronate. Here, we present the crystal structure of human MIOX in complex with myo-inosose-1 bound in a terminal mode to the MIOX diiron cluster site. Furthermore, from biochemical and biophysical results from N-terminal deletion mutagenesis we show that the N terminus is important, through coordination of a set of loops covering the active site, in shielding the active site during catalysis. EPR spectroscopy of the unliganded enzyme displays a two-component spectrum that we can relate to an open and a closed active site conformation. Furthermore, based on site-directed mutagenesis in combination with biochemical and biophysical data, we propose a novel role for Lys(127) in governing access to the diiron cluster.

Structural and Biophysical Characterization of Human myo-Inositol Oxygenase. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Structural and Biophysical Characterization of Human myo-Inositol Oxygenase. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of biological chemistry

VOLUME: 283

Page Numbers: 15209-16

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 24

MONTH: 03

YEAR: 2008

Structural and Biophysical Characterization of Human myo-Inositol Oxygenase. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Structural and Biophysical Characterization of Human myo-Inositol Oxygenase. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Structural and Biophysical Characterization of Human myo-Inositol Oxygenase. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Structural and Biophysical Characterization of Human myo-Inositol Oxygenase.

AFFILIATION: Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, SE-171 77 Stockholm, Sweden.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Structural and Biophysical Characterization of Human myo-Inositol Oxygenase Related Publications

• A new highly asymmetric chelation-controlled heck arylation.
• A reduced repair efficiency can explain increasing melanoma rates.
• A reflux-preventing valve in jejunoileal bypass.
• A survey of possible surgical methods of treating obesity.
• Abscess of the cerebellar lobe of otogenic origin; combined otolaryngologic and neurosurgical treatment in six cases.
• Allowing for the Opposite: The Parents of Asthmatic Children Cooperate by Making Use of Each Other's Differences.
• Central pre- and postsynaptic monoamine receptors in antidepressant therapy.
• ELIMINATION OF INTRAVENOUS FAT FROM THE BLOOD STREAM OF THE DOG.
• Effect of size and position on chest lesion detection.
• Elimination of exogenous lipids from the blood stream. An experimental, methodological and clinical study.
• Functional ability and health complaints among older people with a combination of public and informal care vs. public care only.
• Hospital admissions among people 65+ related to multimorbidity, municipal and outpatient care.
• Impact of delirium on professionals.
• Increasing incidence of brain tumours in sparsely populated areas.
• Iron status of 38-year-old women in Gothenburg, Sweden.
• Life Satisfaction in 6 European Countries: The Relationship to Health, Self-Esteem, and Social and Financial Resources among People (Aged 65-89) with Reduced Functional Capacity.
• Lymphocyte markers in newborn infants.
• Myasthenia gravis. Immunological studies in a young child treated with thymectomy and immunosuppressive drugs.
• Neuropeptides in hyperthermia.
• Older women and men with urinary symptoms.
• Phosphorylation-independent interaction between 14-3-3 and exoenzyme S: from structure to pathogenesis.
• Presentation of amino acid changes. A review.
• Small peptides mimicking substance P (1-7) and encompassing a C-terminal amide functionality.
• Structural and Biophysical Characterization of Human myo-Inositol Oxygenase.
• Studies in lead poisoning. Oral therapy with penicillamine: relationship between lead in blood and other laboratory tests.
• The economic implications of users willingness to increase knowledge capital in health informatics.
• The importance of bioavailability of dietary iron in relation to the expected effect from iron fortification.
• The relative importance of various laboratory measurements in the diagnosis of iron deficiency.
• Wheat fiber, phytates and iron absorption.
• [PLAN FOR THE INCREASE OF MEDICAL SCHOOLS IN NORWAY, 1966-1980.]