Structural analysis of viral nucleocapsids by subtraction of partial projections.

Structural analysis of viral nucleocapsids by subtraction of partial projections. Research Abstract Details 

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Structural analysis of viral nucleocapsids by subtraction of partial projections. Abstract Text:

Ying Zhang,Victor A Kostyuchenko,Michael G Rossmann,

The nucleocapsid of flavivirus particles does not have a recognizable capsid structure when using icosahedral averaging for cryo-electron microscopy structure determinations. The apparent absence of a definitive capsid structure could be due to a lack of synchronization of the symmetry elements of the external glycoprotein layer with those of the core or because the nucleocapsid does not have the same structure within each particle. A technique has been developed to determine the structure of the capsid, and possibly also of the genome, for icosahedral viruses, such as flaviviruses, using cryo-electron microscopy. The method is applicable not only to the analyses of viral cores, but also to the missing structure of multi-component complexes due to symmetry mismatches. The density contributed by external glycoprotein and membrane layers, derived from previously determined three-dimensional icosahedrally averaged reconstructions, was subtracted from the raw images of the virus particles. The resultant difference images were then used for a three-dimensional reconstruction. After appropriate test data sets were constructed and tested, the procedure was applied to examine the nucleocapsids of flaviviruses, which showed that there is no distinct protein density surrounding the genome. Furthermore, there was no evidence of any icosahedral symmetry within the nucleocapsid core.

Structural analysis of viral nucleocapsids by subtraction of partial projections. Publishing Authors By Initials

Y Zhang,VA Kostyuchenko,MG Rossmann,

For similar viruses: virion research abstracts see: viruses: virion research

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Structural analysis of viral nucleocapsids by subtraction of partial projections. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of structural biology

VOLUME: 157

Page Numbers: 356-64

Journal Abbreviation: J. Struct. Biol.

ISSN: 1047-8477

DAY: 16

MONTH: 09

YEAR: 2006

Structural analysis of viral nucleocapsids by subtraction of partial projections. Information

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LANGUAGE: eng

NlmUniqueID: 9011206

Structural analysis of viral nucleocapsids by subtraction of partial projections. Keywords Mesh Terms:

KEYWORDS: Virion

MESH TERMS: chemistry

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Grant and Affiliation Information for Structural analysis of viral nucleocapsids by subtraction of partial projections.

AFFILIATION: Department of Biological Sciences, Purdue University, 915 W. State Street, West Lafayette, IN 47907-2054, USA.

Country: United States

AGENCY: United States NIAID

GRANT: P01 AI055672-010004

ACRONYM: AI

MEDLINETA: J Struct Biol

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