Structural analysis of O-linked sugar chains in human blood clotting factor IX.

Structural analysis of O-linked sugar chains in human blood clotting factor IX. Research Abstract Details 

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Structural analysis of O-linked sugar chains in human blood clotting factor IX. Abstract Text:

N Kuraya,K Omichi,H Nishimura,S Iwanaga,S Hase,

Type and structural analysis of O-linked sugar chains in human blood clotting factor IX was performed by the pyridylamination method developed for O-linked sugar chains [Kuraya, N. & Hase, S. (1992) J. Biochem. 112, 122-126]. O- and N-linked sugar chains were released with hydrazine, and then N-acetylated, followed by pyridylamination. The type of sugar chain was determined by reducing-end analysis of the pyridylaminated (PA-) sugar chains. Sugar chains with PA-GalNAc at the reducing terminal and that with PA-Fuc [Nishimura, H. et al. (1992) J. Biol. Chem. 267, 17520-17525] were obtained besides known sugar chains with PA-Glc from the Xyl-Glc-Ser type and those with PA-GlcNAc from asparagine-linked sugar chains. The sugar chains with PA-GalNAc were identified as mono- and disialyl Gal beta 1-3GalNAc by two-dimensional HPLC mapping. The structure of the sugar chain with PA-Fuc was Neu5Ac alpha 2-6Gal beta 1-4GlcNAc beta 1-3Fuc, as determined by exoglycosidase digestion, methylation analysis, and Smith degradation.

Structural analysis of O-linked sugar chains in human blood clotting factor IX. Publishing Authors By Initials

N Kuraya,K Omichi,H Nishimura,S Iwanaga,S Hase,

For similar carbohydrates: polysaccharides: oligosaccharides research abstracts see: carbohydrates: polysaccharides: oligosaccharides research

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Structural analysis of O-linked sugar chains in human blood clotting factor IX. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 114

Page Numbers: 763-5

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1993

Structural analysis of O-linked sugar chains in human blood clotting factor IX. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Structural analysis of O-linked sugar chains in human blood clotting factor IX. Keywords Mesh Terms:

KEYWORDS: Oligosaccharides

MESH TERMS: chemistry

Chemical & Substance for Abstract: Structural analysis of O-linked sugar chains in human blood clotting factor IX. Information

Substance Name: Factor IX

Registry Number: 9001-28-9

Grant and Affiliation Information for Structural analysis of O-linked sugar chains in human blood clotting factor IX.

AFFILIATION: Department of Chemistry, Osaka University College of Science.

Country: JAPAN

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MEDLINETA: J Biochem

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