Structural analysis of nucleic acids by precise denaturing gradient gel electrophoresis: I. Methodology.

Structural analysis of nucleic acids by precise denaturing gradient gel electrophoresis: I. Methodology. Research Abstract Details 

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Structural analysis of nucleic acids by precise denaturing gradient gel electrophoresis: I. Methodology. Abstract Text:

K Nishigaki,M Tsubota,T Miura,Y Chonan,Y Husimi,

A method to convert the conventional denaturing gradient gel electrophoresis into a highly reproducible experimental system was developed. It was based on the following experimental findings; (i) dyes, which are small molecules, do not exhibit mobility changes attributed to their conformational change while nucleic acids do; and (ii) most of the mobility shifts caused by experimental fluctuations could be cancelled by normalizing the mobility of a sample with respect to the corresponding one of a dye. The method involves co-migration of internal reference dyes with samples (nucleic acids), and computer-aided data processing, allowing us to obtain the relative mobility of nucleic acids with respect to a dye throughout the denaturing gradient. The overall pattern of the relative mobilities thus obtained, named the normalized mobility profile (NMP), corresponded well to conformational changes of a macromolecule induced by denaturing effects. This method provides us with objective data without using internal macromolecular references, which not only guarantees the precision but also extends the range of application of the denaturing gradient method.

Structural analysis of nucleic acids by precise denaturing gradient gel electrophoresis: I. Methodology. Publishing Authors By Initials

K Nishigaki,M Tsubota,T Miura,Y Chonan,Y Husimi,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids research

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Structural analysis of nucleic acids by precise denaturing gradient gel electrophoresis: I. Methodology. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 111

Page Numbers: 144-50

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1992

Structural analysis of nucleic acids by precise denaturing gradient gel electrophoresis: I. Methodology. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Structural analysis of nucleic acids by precise denaturing gradient gel electrophoresis: I. Methodology. Keywords Mesh Terms:

KEYWORDS: Nucleic Acids

MESH TERMS: chemistry

Chemical & Substance for Abstract: Structural analysis of nucleic acids by precise denaturing gradient gel electrophoresis: I. Methodology. Information

Substance Name: Nucleic Acids

Registry Number: 0

Grant and Affiliation Information for Structural analysis of nucleic acids by precise denaturing gradient gel electrophoresis: I. Methodology.

AFFILIATION: Department of Environmental Chemistry, Faculty of Engineering, Saitama University.

Country: JAPAN

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MEDLINETA: J Biochem

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Structural analysis of nucleic acids by precise denaturing gradient gel electrophoresis: I Methodology Related Publications

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• Structural analysis of nucleic acids by precise denaturing gradient gel electrophoresis: I. Methodology.
• Structural analysis of nucleic acids by precise denaturing gradient gel electrophoresis: II. Applications to the analysis of subtle and drastic mobility changes of oligo- and polynucleotides.