Stretch Induction of Cyclooxygenase-2 Expression in Human Urothelial Cells Is Calcium- and Protein Kinase C {zeta}-Dependent.

Stretch Induction of Cyclooxygenase-2 Expression in Human Urothelial Cells Is Calcium- and Protein Kinase C {zeta}-Dependent. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Stretch Induction of Cyclooxygenase-2 Expression in Human Urothelial Cells Is Calcium- and Protein Kinase C {zeta}-Dependent. Abstract Text:

Travis J Jerde,William S Mellon,Dale E Bjorling,Celina M Checura,Kwadwo Owusu-Ofori,John J Parrish,Stephen Y Nakada,Travis J Jerde,William S Mellon,Dale E Bjorling,Celina M Checura,Kwadwo Owusu-Ofori,John J Parrish,Stephen Y Nakada,Travis J Jerde,William S Mellon,Dale E Bjorling,Celina M Checura,Kwadwo Owusu-Ofori,John J Parrish,Stephen Y Nakada,Travis J Jerde,William S Mellon,Dale E Bjorling,Celina M Checura,Kwadwo Owusu-Ofori,John J Parrish,Stephen Y Nakada,

Prostanoid synthesis via cyclooxygenase (COX)-2 induction during urothelial stretch is central to nociception, inflammation, contractility, and proliferation caused by urinary tract obstruction. We used our primary human urothelial cell stretch model published previously to evaluate the signaling mechanisms responsible for stretch-induced COX-2 expression in urothelial cells. To determine intracytosolic calcium concentrations ([Ca(2+)](i)), primary human urothelial cells were grown on flexible membranes and loaded with Fura-2 acetoxymethyl ester (AM). We determined [Ca(2+)](i) using a fluorescent scope during stretch. Additional cells were treated with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA)-AM, stretched, and COX-2 mRNA and protein were evaluated by real-time polymerase chain reaction and immunoblotting. To evaluate protein kinase C (PKC) in this system, cells were stretched and fractionated into membrane, cytosol, and nucleus. Fractions were immunoblotted for PKCalpha, beta1, and zeta, the predominant isoforms in urothelial cells. We treated additional cells with increasing concentrations of either bisindolylmaleimide-I or a peptide PKC pseudosubstrate inhibitor, and COX-2 mRNA and protein were evaluated after stretching. Furthermore, we transfected urothelial cells with siRNA against each of the inducible PKC isoforms in these cells and evaluated the stretch-induced COX-2 response. Stretch of urothelial cells activated calcium flux and PKC translocation to membrane and nucleus. Pharmacological inhibition indicated that stretch-induced COX-2 expression is dependent on calcium and PKC, and biochemical knockdown experiments indicated that PKCzeta is the predominant isoform mediating stretch-induced COX-2 expression. Elucidating the signaling mechanism of stretch-induced COX-2 expression may identify therapeutic targets.

Stretch Induction of Cyclooxygenase-2 Expression in Human Urothelial Cells Is Calcium- and Protein Kinase C {zeta}-Dependent. Publishing Authors By Initials

TJ Jerde,WS Mellon,DE Bjorling,CM Checura,K Owusu-Ofori,JJ Parrish,SY Nakada,TJ Jerde,WS Mellon,DE Bjorling,CM Checura,K Owusu-Ofori,JJ Parrish,SY Nakada,TJ Jerde,WS Mellon,DE Bjorling,CM Checura,K Owusu-Ofori,JJ Parrish,SY Nakada,TJ Jerde,WS Mellon,DE Bjorling,CM Checura,K Owusu-Ofori,JJ Parrish,SY Nakada,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Stretch Induction of Cyclooxygenase-2 Expression in Human Urothelial Cells Is Calcium- and Protein Kinase C {zeta}-Dependent. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Molecular pharmacology

VOLUME: 73

Page Numbers: 18-26

Journal Abbreviation: Mol. Pharmacol.

ISSN: 1521-0111

DAY: 20

MONTH: Jan

YEAR: 2008

Stretch Induction of Cyclooxygenase-2 Expression in Human Urothelial Cells Is Calcium- and Protein Kinase C {zeta}-Dependent. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 35623

Stretch Induction of Cyclooxygenase-2 Expression in Human Urothelial Cells Is Calcium- and Protein Kinase C {zeta}-Dependent. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Stretch Induction of Cyclooxygenase-2 Expression in Human Urothelial Cells Is Calcium- and Protein Kinase C {zeta}-Dependent. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Stretch Induction of Cyclooxygenase-2 Expression in Human Urothelial Cells Is Calcium- and Protein Kinase C {zeta}-Dependent.

AFFILIATION: University of Wisconsin Medical School, Department of Surgery, Division of Urology, K6-561 Clinical Science Center; 600 Highland Avenue, Madison, WI 53792. jerde@surgery.wisc.edu.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Mol Pharmacol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Stretch Induction of Cyclooxygenase-2 Expression in Human Urothelial Cells Is Calcium- and Protein Kinase C {zeta}-Dependent Related Publications

• An in vivo study of the replication origin in the influenza virus complementary RNA.
• Asymmetric catalysis of Nozaki-Hiyama allylation and methallylation with a new tridentate bis(oxazolinyl)carbazole ligand.
• Asymmetric catalysis on the intramolecular cyclopropanation of alpha-diazo-beta-keto sulfones.
• Biomimetic total synthesis of (-)-erinacine e.
• Cryoablation of small renal masses: assessment of the outcome at one institution.
• Current practice patterns in the use of ablation technology for the management of small renal masses at academic centers in the United States.
• Editorial comment.
• Glycosylation of the tandem repeat unit of the MUC2 polypeptide leading to the synthesis of the Tn antigen.
• Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes.
• Health related quality of life differs between male and female stone formers.
• High density O-glycosylation of the MUC2 tandem repeat unit by N-acetylgalactosaminyltransferase-3 in colonic adenocarcinoma extracts.
• Lemonade therapy increases urinary citrate and urine volumes in patients with recurrent calcium oxalate stone formation.
• Oncological outcomes of hand-assisted laparoscopic radical nephrectomy for clinically localized renal cell carcinoma: a single-institution study with >or=3 years of follow-up.
• Purification of fibrinogen using cationic detergent.
• Serial assessments of delayed encephalopathy after carbon monoxide poisoning using magnetic resonance spectroscopy and diffusion tensor imaging on 3.0T system.
• Spin projection in the shell model monte carlo method and the spin distribution of nuclear level densities.
• Stretch Induction of Cyclooxygenase-2 Expression in Human Urothelial Cells Is Calcium- and Protein Kinase C {zeta}-Dependent.
• Thymine starvation and beta-galactosidase synthesis.
• [A case report on giant benign localized pleural fibroma (author's transl)]
• [Achievement of the home health service section of our hospital in the past 5 years--the third function of the hospital]
• [Clinical features of patients with recurrent Kawasaki disease]
• [Esophago-bronchial fistula associated with intralobar pulmonary sequestration. A case report (author's transl)]
• [Gamma knife radiosurgery for temporal bone chondroblastoma: case report]
• [Immunologic diagnosis of graft rejection in lung allotransplantation]
• [Improved technique for one-stage bilateral lung autotransplanation as a model of total lung denervation (author's transl)]
• [Nursing report: an innovation on a simple holder for perfusion fluid used in TUR (transurethral resection)]
• [Pneumonectomy and postoperative care of patients with depressed lung function]
• [Postoperative pulmonary complications and their prevention following surgery of lung cancer]
• [Thymoma-clinical disorders and results of surgical treatment (author's transl)]
• beta-Galactosidase synthesis by Escherichia coli zygotes in the absence of DNA synthesis.