Stress-induced c-Jun activation mediated by Polo-like kinase 3 in corneal epithelial cells.

Stress-induced c-Jun activation mediated by Polo-like kinase 3 in corneal epithelial cells. Research Abstract Details 

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Stress-induced c-Jun activation mediated by Polo-like kinase 3 in corneal epithelial cells. Abstract Text:

Ling Wang,Wei Dai,Luo Lu,Ling Wang,Wei Dai,Luo Lu,

Polo-like kinase 3 (Plk3) activation occurs after exposure to environmental or genotoxic stresses. Plk3 regulates cell fate through regulating cell cycle progression. UV irradiation is one of the major environmental stresses that affect corneal epithelial wound healing. In the present study, we report that UV irradiation activated Plk3 and that Plk3 interacts with AP-1 and c-Jun, which appears to be important to mediate corneal epithelial cell apoptosis after UV irradiation. Recombinant Plk3, as well as Plk3 immunoprecipitated from UV-irradiated cells, phosphorylated c-Jun in vitro. The phosphorylation of c-Jun by Plk3 immunoprecipitates was not altered by the pre-removal of JNK from the cell lysates. In addition, the effect of UV irradiation-induced phosphorylation of c-Jun and apoptosis were not significantly affected by knockdown of JNK mRNA. Co-immunoprecipitation reveals that Plk3 and c-Jun directly interacted with each other. Consistently, Plk3 co-localized with c-Jun to the nucleus after UV irradiation. Further, modulating Plk3 activities by overexpressing Plk3 or its mutants significantly affected UV irradiation-induced c-Jun activity and subsequent apoptosis. Our results thus provide for the first time that Plk3 mediates UV irradiation-induced c-Jun activation by phosphorylating c-Jun, suggesting that Plk3 plays an important role in mediating programmed cell death of corneal epithelial cells after UV irradiation.

Stress-induced c-Jun activation mediated by Polo-like kinase 3 in corneal epithelial cells. Publishing Authors By Initials

L Wang,W Dai,L Lu,L Wang,W Dai,L Lu,

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Stress-induced c-Jun activation mediated by Polo-like kinase 3 in corneal epithelial cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 32121-7

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 5

MONTH: 09

YEAR: 2007

Stress-induced c-Jun activation mediated by Polo-like kinase 3 in corneal epithelial cells. Information

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LANGUAGE: eng

NlmUniqueID: 2985121

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Grant and Affiliation Information for Stress-induced c-Jun activation mediated by Polo-like kinase 3 in corneal epithelial cells.

AFFILIATION: Department of Medicine, Harbor-UCLA Medical Center, David Geffen School of Medicine, University of California Los Angeles, Torrance, California 90502, USA.

Country: United States

AGENCY: United States NEI

GRANT: EY12953

ACRONYM: EY

MEDLINETA: J Biol Chem

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