Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost.

Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost. Research Abstract Details 

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Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost. Abstract Text:

Wolfgang Peti,Rebecca Page,

Automation and miniaturization are key issues of high-throughput research projects in the post-genomic era. The implementation of robotics and parallelization has enabled researchers to process large numbers of protein targets for structural studies in a short time with reasonable cost efficiency. However, the cost of implementing the robotics and parallelization often prohibit their use in the traditional academic laboratory. Fortunately, multiple groups have made significant efforts to minimize the cost of heterologous protein expression for the production of protein samples in quantities suitable for high resolution structural studies. In this review, we describe recent efforts to continue to minimize the cost for the parallel processing of multiple protein targets and focus on those materials and strategies that are highly suitable for the traditional academic laboratory.

Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost. Publishing Authors By Initials

W Peti,R Page,

For similar natural sciences: electronics: robotics research abstracts see: natural sciences: electronics: robotics research

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Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost. Journal Published:

PUBLICATION TYPE: Review

Journal: Protein expression and purification

VOLUME: 51

Page Numbers: 1-10

Journal Abbreviation: Protein Expr. Purif.

ISSN: 1046-5928

DAY: 4

MONTH: 07

YEAR: 2006

Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost. Information

Number of References: 47

LANGUAGE: eng

NlmUniqueID: 9101496

Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost. Keywords Mesh Terms:

KEYWORDS: Robotics

MESH TERMS: economics

Chemical & Substance for Abstract: Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost. Information

Substance Name: Recombinant Fusion Proteins

Registry Number: 0

Grant and Affiliation Information for Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost.

AFFILIATION: Brown University, Department of Molecular Pharmacology, Physiology, and Biotechnology, Box G-E3, Providence, RI 02912, USA.

Country: United States

AGENCY: United States NIBIB

GRANT: R01 EB003872-02

ACRONYM: EB

MEDLINETA: Protein Expr Purif

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