Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity.

Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity. Abstract Text:

Po Yu Chen,Lasse Weinmann,Dimos Gaidatzis,Yi Pei,Mihaela Zavolan,Thomas Tuschl,Gunter Meister,Po Yu Chen,Lasse Weinmann,Dimos Gaidatzis,Yi Pei,Mihaela Zavolan,Thomas Tuschl,Gunter Meister,

Small interfering RNAs (siRNAs) and microRNAs (miRNAs) guide catalytic sequence-specific cleavage of fully or nearly fully complementary target mRNAs or control translation and/or stability of many mRNAs that share 6-8 nucleotides (nt) of complementarity to the siRNA and miRNA 5' end. siRNA- and miRNA-containing ribonucleoprotein silencing complexes are assembled from double-stranded 21- to 23-nt RNase III processing intermediates that carry 5' phosphates and 2-nt overhangs with free 3' hydroxyl groups. Despite the structural symmetry of a duplex siRNA, the nucleotide sequence asymmetry can generate a bias for preferred loading of one of the two duplex-forming strands into the RNA-induced silencing complex (RISC). Here we show that the 5'-phosphorylation status of the siRNA strands also acts as an important determinant for strand selection. 5'-O-methylated siRNA duplexes refractory to 5' phosphorylation were examined for their biases in siRNA strand selection. Asymmetric, single methylation of siRNA duplexes reduced the occupancy of the silencing complex by the methylated strand with concomitant elimination of its off-targeting signature and enhanced off-targeting signature of the phosphorylated strand. Methylation of both siRNA strands reduced but did not completely abolish RNA silencing, without affecting strand selection relative to that of the unmodified siRNA. We conclude that asymmetric 5' modification of siRNA duplexes can be useful for controlling targeting specificity.

Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity. Publishing Authors By Initials

PY Chen,L Weinmann,D Gaidatzis,Y Pei,M Zavolan,T Tuschl,G Meister,PY Chen,L Weinmann,D Gaidatzis,Y Pei,M Zavolan,T Tuschl,G Meister,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: RNA (New York, N.Y.)

VOLUME: 14

Page Numbers: 263-74

Journal Abbreviation: RNA

ISSN: 1469-9001

DAY: 19

MONTH: 12

YEAR: 2007

Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9509184

Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity.

AFFILIATION:

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: RNA

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity Related Publications

• A neural computation for visual acuity in the presence of eye movements.
• Adrenergic receptors and sodium reabsorption in normotensive subjects as related to salt sensitivity.
• Applications of human reliability to the production process. AMRL-TR-67-88.
• Classification, grading, and staging of soft tissue sarcomas.
• Comparative study of figure-ground discrimination in preschool children and adults.
• Compensation of the bias caused by the wall filter on the mean Doppler frequency.
• Differences in Scapular Upward Rotation between Baseball Pitchers and Position Players: 2515: Board #62 June 2 8:00 AM - 9:30 AM.
• Differences in scapular upward rotation between baseball pitchers and position players.
• Enzymatic degradation of triacetic acid lactone determined by a spectrophotometric method.
• From program to policy: expanding the role of community coalitions.
• Identification of human microRNA targets from isolated argonaute protein complexes.
• Lactonase and C-acylase activity of hepatoma.
• Metabolism of 3,5-diketohexanoic acid and its delta-lactone by tissue homogenates.
• Neurotransmitters in key neurons of the hypothalamus that regulate feeding behavior and body weight.
• Participation: of phenylacetyl-adenylate in the enzymic synthesis of phenylacetylglutamine.
• Polycystic disease of the liver; report of two cases diagnosed by peritoneoscopy.
• Presence of pituitary adenylate cyclase-activating polypeptide (PACAP) defines a subpopulation of hypothalamic POMC neurons.
• Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.
• Retinal adaptation to object motion.
• Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity.
• Studies on d- and tau-alpha-keto-beta-methylvaleric acids.
• [Causes of suture aneurysms]
• [Echinococcosis today--dawn of effective drug therapy]
• [In Process Citation]
• [J-pouch--the Erlangen experience]
• [Not Available.]
• [Present possibilities of antibiotic therapy in chronic non-specific respiratory diseases (author's transl)]
• [Residual cancer or not residual cancer--that is the question]
• [Suggestions for allergological diagnostics in outpatients' departments for lung diseases and tuberculosis (author's transl)]
• miRNAs get an early start on translational silencing.