Stopped-flow studies on the chemical modification with N-bromosuccinimide of model compounds of tryptophan residues.

Stopped-flow studies on the chemical modification with N-bromosuccinimide of model compounds of tryptophan residues. Research Abstract Details 

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Stopped-flow studies on the chemical modification with N-bromosuccinimide of model compounds of tryptophan residues. Abstract Text:

M Ohnishi,T Kawagishi,T Abe,K Hiromi,

The aim of this work was to study the selective modification of tryptophan residues with NBS. To accomplish this, a specific method to determine tryptophan was required initially. NBS reacts with both tryptophan and tyrosine residues, which are found in most enzyme proteins, and static spectrophotometric observation, which is usually employed to follow the progress of modification, is not selective for tryptophan. However, discrimination of tryptophan from tyrosine was achieved by the kinetic method with a stoppeed-flow apparatus. The rate of modification of tryptophan residues is 10(3) times larger than that of tyrosine, so rapid stopping of the reaction of NBS brings about the selective modification of tryptophan residues. Using fluorescence-spectrophotometric and kinetic methods, the modification with NBS of model compounds of the tryptophan residue could be simply followed as a single phase, even though the reaction is complex when followed by the static spectrophotometric method.

Stopped-flow studies on the chemical modification with N-bromosuccinimide of model compounds of tryptophan residues. Publishing Authors By Initials

M Ohnishi,T Kawagishi,T Abe,K Hiromi,

For similar amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tyrosine research abstracts see: amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tyrosine research

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Stopped-flow studies on the chemical modification with N-bromosuccinimide of model compounds of tryptophan residues. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 87

Page Numbers: 273-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1980

Stopped-flow studies on the chemical modification with N-bromosuccinimide of model compounds of tryptophan residues. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Stopped-flow studies on the chemical modification with N-bromosuccinimide of model compounds of tryptophan residues. Keywords Mesh Terms:

KEYWORDS: Tyrosine

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Chemical & Substance for Abstract: Stopped-flow studies on the chemical modification with N-bromosuccinimide of model compounds of tryptophan residues. Information

Substance Name: Tryptophan

Registry Number: 73-22-3

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Country: JAPAN

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MEDLINETA: J Biochem

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