Stimulation by aminoacyl-tRNA of the GTPase and ATPase activities of rat liver 5S RNA protein particles in the presence of EF-2.

Stimulation by aminoacyl-tRNA of the GTPase and ATPase activities of rat liver 5S RNA protein particles in the presence of EF-2. Research Abstract Details 

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Stimulation by aminoacyl-tRNA of the GTPase and ATPase activities of rat liver 5S RNA protein particles in the presence of EF-2. Abstract Text:

K Ogata,K Terao,T Uchiumi,

The GTP- and ATP-hydrolyzing activities of the rat liver 5S RNA-L5 (according to the proposed common nomenclature (1) protein complex designated as 5S RNP were stimulated by pig liver elongation factor 2 (EF-2) plus aminoacyl-tRNA, both of which were required for the stimulation. The stimulative effect of aminoacyl-tRNA on GTP hydrolysis by the complete system containing 5S RNP and EF-2 was dependent on the concentration of aminoacyl-tRNA. Aminoacyl-tRNA also stimulated ATP hydrolysis by the complete system but did not stimulate the ATP hydrolysis by 5S RNP alone or EF-2 alone. While deacylated tRNA stimulated the ATPase activity of the complete system, it had no effect on the GTPase activity. Preincubated tRNA lacking the 3'-terminal CCA moiety had little effect on the GTPase and ATPase activities of the complete system. Fusidic acid inhibited the GTPase and the ATPase activities of the complete system with and without aminoacyl-tRNA, although the extent of the inhibition was larger in the presence of aminoacyl-tRNA. These results suggest that 5S RNP may be a component of the GTPase center of rat liver 60S subunits.

Stimulation by aminoacyl-tRNA of the GTPase and ATPase activities of rat liver 5S RNA protein particles in the presence of EF-2. Publishing Authors By Initials

K Ogata,K Terao,T Uchiumi,

For similar chemical and pharmacologic phenomena: stimulation, chemical research abstracts see: chemical and pharmacologic phenomena: stimulation, chemical research

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Stimulation by aminoacyl-tRNA of the GTPase and ATPase activities of rat liver 5S RNA protein particles in the presence of EF-2. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 87

Page Numbers: 517-24

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1980

Stimulation by aminoacyl-tRNA of the GTPase and ATPase activities of rat liver 5S RNA protein particles in the presence of EF-2. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Stimulation by aminoacyl-tRNA of the GTPase and ATPase activities of rat liver 5S RNA protein particles in the presence of EF-2. Keywords Mesh Terms:

KEYWORDS: Stimulation, Chemical

MESH TERMS: metabolism

Chemical & Substance for Abstract: Stimulation by aminoacyl-tRNA of the GTPase and ATPase activities of rat liver 5S RNA protein particles in the presence of EF-2. Information

Substance Name: GTP Phosphohydrolase-Linked Elongation F

Registry Number: EC 3.6.1.-

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Country: JAPAN

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MEDLINETA: J Biochem

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