Steroids conjugated to bovine serum albumin as tools to demonstrate specific steroid neuronal membrane binding sites.

Steroids conjugated to bovine serum albumin as tools to demonstrate specific steroid neuronal membrane binding sites. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Steroids conjugated to bovine serum albumin as tools to demonstrate specific steroid neuronal membrane binding sites. Abstract Text:

Our laboratory has pioneered the use of bovine serum albumin (BSA) linked to different positions of the ring structure of progesterone to investigate steroid-membrane interactions. The complex can be radioiodinated to demonstrate the existence of specific membrane progesterone binding sites in the rat brain. Not only are these progesterone complexes specific ligands, but they also elicit functional responses in the central nervous system (CNS), particularly in the corpus striatum (CS) where progesterone-BSA conjugates linked at C-3 and C-11 positions (P-3-BSA and P-11-BSA) alter amphetamine-evoked dopamine release. In this communication we will report our current studies that use radioiodinated progesterone-BSA conjugates (P-3-125I-BSA, P-6-125I-BSA, and P-11-125I-BSA) and estradiol-BSA conjugates linked at C-6 position (17 beta-E-6-125I-BSA and 17-E-6-BSA) to demonstrate the existence of specific membrane binding sites for progesterone and estrogen in several regions of the rat brain. In addition, initial studies to isolate and purify these membrane binding sites from digitonin-solubilized P2-membrane fractions by affinity chromatography are reported. The data indicate that these sites are part of a complex membrane receptor for either estrogen or progesterone, the so-called membrane estrogen receptor (mER) and the membrane progesterone receptor (mPR), respectively.

Steroids conjugated to bovine serum albumin as tools to demonstrate specific steroid neuronal membrane binding sites. Publishing Authors By Initials

For similar nervous system: synapses: synaptic membranes research abstracts see: nervous system: synapses: synaptic membranes research

PUBMED ID PMID:

MEDLINE DATE:

Steroids conjugated to bovine serum albumin as tools to demonstrate specific steroid neuronal membrane binding sites. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of psychiatry & neuroscience : JPN

VOLUME: 21

Page Numbers: 187-97

Journal Abbreviation: J Psychiatry Neurosci

ISSN: 1180-4882

DAY: 6

MONTH: May

YEAR: 1996

Steroids conjugated to bovine serum albumin as tools to demonstrate specific steroid neuronal membrane binding sites. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9107859

Steroids conjugated to bovine serum albumin as tools to demonstrate specific steroid neuronal membrane binding sites. Keywords Mesh Terms:

KEYWORDS: Synaptic Membranes

MESH TERMS: physiology

Chemical & Substance for Abstract: Steroids conjugated to bovine serum albumin as tools to demonstrate specific steroid neuronal membrane binding sites. Information

Substance Name: Progesterone

Registry Number: 57-83-0

Grant and Affiliation Information for Steroids conjugated to bovine serum albumin as tools to demonstrate specific steroid neuronal membrane binding sites.

AFFILIATION: Neuroscience Program, University of Illinois, Urbana 61801, USA.

Country: CANADA

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Psychiatry Neurosci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Steroids conjugated to bovine serum albumin as tools to demonstrate specific steroid neuronal membrane binding sites Related Publications

• A process for analysis of sentinel events due to health care-associated infection.
• Adenosquamous carcinoma versus adenocarcinoma in early-stage cervical cancer patients undergoing radical hysterectomy: an outcomes analysis.
• Arsenite induced oxidative damage in mouse liver is associated with increased cytokeratin 18 expression.
• Basolateral amygdala inactivation by muscimol, but not ERK/MAPK inhibition, impairs the use of reward expectancies during working memory.
• Characterization of the mid-foregut transcriptome identifies genes regulated during lung bud induction.
• Diagnosis of short cervix in a community hospital prior to placement of a therapeutic cerclage.
• Effect of friction between particles in the dynamic response of model magnetic structures.
• Establishing a hospital program to improve antimicrobial use, control bacterial resistance and contain healthcare costs: the University of Louisville experience.
• Fostering international multicenter collaborative research: the CAPO Project.
• Gender symmetry in prevalence, severity, and chronicity of physical aggression against dating partners by university students in Mexico and USA.
• Interstitial cells of Cajal in patients with chagasic megacolon originating from a region of old endemicity.
• Invited discussion: midface lifting as an adjunct procedure in lower eyelid ectropion repair.
• Letter to the editor.
• Manufacturing heterosexuality: hormone replacement therapy and menopause in urban Oaxaca.
• Measurement of the eta-meson mass using psi(2S) --> etaJ/psi.
• Moore or less the truth? "Sicko".
• Multiple pathways regulate intracellular shuttling of MoKA, a co-activator of transcription factor KLF7.
• Prevalence and socio-demographic correlates of drug use among adolescents: results from the Mexican Adolescent Mental Health Survey.
• Progesterone levels and relationship with the diagnosis of a corpus luteum by rectal palpation during the estrous cycle in Zebu cows.
• Receptor mechanism and antiemetic activity of structurally-diverse cannabinoids against radiation-induced emesis in the least shrew.
• Reevaluating the serotype II capsular locus of Streptococcus agalactiae.
• Reperfusion seizures: a manifestation of cerebral reperfusion injury after administration of recombinant tissue plasminogen activator for acute ischemic stroke.
• Steroids with a carbamate function at C-17, a novel class of inhibitors for human and hamster steroid 5alpha-reductase.
• The first intron of the human alpha2(I) collagen gene (COL1A2) contains a novel interferon-gamma responsive element.
• Vascular oxidative stress is associated with insulin resistance in hyper-reninemic nonmodulating essential hypertension.
• [A 50-year-old male with AIDS and retroperitoneal mass]
• [Gender perspective in health care teaching: A pending task.]
• [Incidence and mortality of massive transfusion in a university hospital: study of the period 2001-2005]
• [Not Available.]
• [Not Available.]