Steric Block High Affinity Oligonucleotide Analogues: A New Tool for Mapping RNA-Protein Binding Sites.

Steric Block High Affinity Oligonucleotide Analogues: A New Tool for Mapping RNA-Protein Binding Sites. Research Abstract Details 

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Steric Block High Affinity Oligonucleotide Analogues: A New Tool for Mapping RNA-Protein Binding Sites. Abstract Text:

Douglas Brown,Emily Joy,Jane Greatorex,Michael J Gait,Andrew M L Lever,

Steric-block ON analogues are efficient inhibitors of RNA-protein interaction and therefore have potential to probe RNA sequences for putative protein binding sites and to investigate mechanisms of protein binding. The packaging process of HIV-1 is highly specific involving an interaction between the Gag protein and a conserved sequence that is only present on genomic viral RNA. Using oligonucleotide probes we have confirmed that the terminal purine loop is the major Gag binding site on SL3 and that a secondary Gag binding site exists at an internal purine bulge. We also demonstrate direct binding of oligonucleotide to their binding sites and confirm this interaction does not alter global RNA conformation, making them highly specific, nondisruptive probes of RNA protein interactions.

Steric Block High Affinity Oligonucleotide Analogues: A New Tool for Mapping RNA-Protein Binding Sites. Publishing Authors By Initials

D Brown,E Joy,J Greatorex,MJ Gait,AM Lever,

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Steric Block High Affinity Oligonucleotide Analogues: A New Tool for Mapping RNA-Protein Binding Sites. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Nucleosides, nucleotides & nucleic acids

VOLUME: 27

Page Numbers: 196-212

Journal Abbreviation: Nucleosides Nucleotides Nuclei

ISSN: 1525-7770

DAY: 21

MONTH: 01

YEAR: 2008

Steric Block High Affinity Oligonucleotide Analogues: A New Tool for Mapping RNA-Protein Binding Sites. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Steric Block High Affinity Oligonucleotide Analogues: A New Tool for Mapping RNA-Protein Binding Sites.

AFFILIATION: Department of Medicine, University of Cambridge, Addenbrookes Hospital, Cambridge, United Kingdom.

Country: United States

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MEDLINETA: Nucleosides Nucleotides Nuclei

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