Stereochemical course of enoyl reduction catalyzed by fatty acid synthetase. Stereochemistry of hydrogen incorporation from reduced pyridine nucleotide.

Stereochemical course of enoyl reduction catalyzed by fatty acid synthetase. Stereochemistry of hydrogen incorporation from reduced pyridine nucleotide. Research Abstract Details 

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Stereochemical course of enoyl reduction catalyzed by fatty acid synthetase. Stereochemistry of hydrogen incorporation from reduced pyridine nucleotide. Abstract Text:

A Kawaguchi,T Yoshimura,K Saito,Y Seyama,T Kasama,T Yamakawa,S Okuda,

The stereochemical course of the enoyl reduction catalyzed by fatty acid synthetase was investigated using the enzymes from rat liver and Brevibacterium ammoniagenes. Deuterium-labeled fatty acids were synthesized by incubating the synthetases with either 4R-[4-2H1]- or 4S-[4-2H1]NADPH. The deuterium-labeled fatty acids thus produced were subjected to the action of a stereospecific enzyme, acyl-CoA oxidase. The deuterium-labeled fatty acids and 2,3-dehydroacyl thioesters, the products of acyl-CoA oxidase, were methylated and analyed for deuterium content by gas chromatography-mass spectrometry. These experiments provided information to determine the configuration at the C-3 position of fatty acids formed by fatty acid synthetases. The results suggested that the stereochemistry of hydrogen (as hydride) incorporation from reduced pyridine nucleotides during enoyl reduction was different between rat liver and B. ammoniagenes synthetases: the enoyl reduction of rat liver enzyme involved the re-attack of hydride and that of B. ammoniagenes enzyme involved the si-attack of hydride.

Stereochemical course of enoyl reduction catalyzed by fatty acid synthetase. Stereochemistry of hydrogen incorporation from reduced pyridine nucleotide. Publishing Authors By Initials

A Kawaguchi,T Yoshimura,K Saito,Y Seyama,T Kasama,T Yamakawa,S Okuda,

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Stereochemical course of enoyl reduction catalyzed by fatty acid synthetase. Stereochemistry of hydrogen incorporation from reduced pyridine nucleotide. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 88

Page Numbers: 1-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 1980

Stereochemical course of enoyl reduction catalyzed by fatty acid synthetase. Stereochemistry of hydrogen incorporation from reduced pyridine nucleotide. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Stereochemical course of enoyl reduction catalyzed by fatty acid synthetase. Stereochemistry of hydrogen incorporation from reduced pyridine nucleotide. Keywords Mesh Terms:

KEYWORDS: Tritium

MESH TERMS: metabolism

Chemical & Substance for Abstract: Stereochemical course of enoyl reduction catalyzed by fatty acid synthetase. Stereochemistry of hydrogen incorporation from reduced pyridine nucleotide. Information

Substance Name: Fatty Acid Synthetase Complex

Registry Number: EC 6.-

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Country: JAPAN

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MEDLINETA: J Biochem

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