Stellate cell apoptosis by a soluble mediator from immortalized human hepatocytes.

Stellate cell apoptosis by a soluble mediator from immortalized human hepatocytes. Research Abstract Details 

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Stellate cell apoptosis by a soluble mediator from immortalized human hepatocytes. Abstract Text:

Arnab Basu,Kousuke Saito,Keith Meyer,Ratna B Ray,Scott L Friedman,Yie-Hwa Chang,Ranjit Ray,

Activated hepatic stellate cells (HSCs) are the major source of extracellular matrix in fibrosis and cirrhosis. In this study, we have investigated the role of hepatitis C virus (HCV) core protein induced immortalized human hepatocytes (IHH) on HSC growth. Preferential growth of IHH and apoptosis of activated human hepatic stellate cells (LX2) were observed upon coculture of these two cell types in a dual chamber or in the presence of conditioned medium (CM) from IHH. CM did not display a growth inhibitory role on other hepatic (Huh-7, HepG2, Hep3B and THLE) and non-hepatic (HeLa, MCF-7, and BHK) epithelial cells, indicating that the soluble mediator from IHH does not have a generalized effect on cell lines examined in our study. Further studies suggested that CM from IHH increased the expression of TRAIL receptors on LX2 cell surface, and induced apoptosis by a caspase dependent mechanism. Peptide mass fingerprinting of the purified soluble mediator from CM suggested that gelsolin fragments may play a role in apoptosis of LX2 cells. Taken together, our results suggested that a soluble mediator secreted from immortalized human hepatocytes plays an important role in hepatic stellate cell growth regulation.

Stellate cell apoptosis by a soluble mediator from immortalized human hepatocytes. Publishing Authors By Initials

A Basu,K Saito,K Meyer,RB Ray,SL Friedman,YH Chang,R Ray,

For similar proteins: viral proteins: viral structural proteins: nucleocapsid proteins: viral core proteins research abstracts see: proteins: viral proteins: viral structural proteins: nucleocapsid proteins: viral core proteins research

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Stellate cell apoptosis by a soluble mediator from immortalized human hepatocytes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Apoptosis : an international journal on programmed

VOLUME: 11

Page Numbers: 1391-400

Journal Abbreviation: Apoptosis

ISSN: 1360-8185

DAY: 3

MONTH: Aug

YEAR: 2006

Stellate cell apoptosis by a soluble mediator from immortalized human hepatocytes. Information

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LANGUAGE: eng

NlmUniqueID: 9712129

Stellate cell apoptosis by a soluble mediator from immortalized human hepatocytes. Keywords Mesh Terms:

KEYWORDS: Viral Core Proteins

MESH TERMS: physiology

Chemical & Substance for Abstract: Stellate cell apoptosis by a soluble mediator from immortalized human hepatocytes. Information

Substance Name: Caspase 8

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Stellate cell apoptosis by a soluble mediator from immortalized human hepatocytes.

AFFILIATION: Department of Internal Medicine, Saint Louis University, St. Louis, MO 63110, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK 56621

ACRONYM: DK

MEDLINETA: Apoptosis

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