Stearoyl-CoA desaturase mRNA expression during bovine adipocyte differentiation in primary culture derived from Japanese Black and Holstein cattle.

Stearoyl-CoA desaturase mRNA expression during bovine adipocyte differentiation in primary culture derived from Japanese Black and Holstein cattle. Research Abstract Details 

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Stearoyl-CoA desaturase mRNA expression during bovine adipocyte differentiation in primary culture derived from Japanese Black and Holstein cattle. Abstract Text:

H Ohsaki,T Sawa,S Sasazaki,K Kano,M Taniguchi,F Mukai,H Mannen,

Stearoyl-CoA desaturase (SCD) catalyzes the synthesis of monounsaturated fatty acids (MUFAs). In cattle, the MUFAs are related to softness and flavor of meat. In order to investigate gene expression profile during bovine preadipocyte differentiation, we isolated stromal-vascular cells from perirenal adipose tissues of Japanese Black and Holstein steers. Gene expression level of adipocyte type fatty acid binding protein (FABP4), SCD, sterol regulatory element binding protein 1 (SREBP1) and CCAAT/enhancer binding protein alpha (C/EBP-alpha) were elucidated by real-time PCR assay. The levels of SCD mRNA expression were significantly increased to 10.8 and 6.3-fold in Japanese Black and Holstein, respectively, on day 1 of the culture. The difference in SCD expression between the two breeds may reflect differences in the fat development characteristics of the cattle breeds. Although transcription factors SREBP1 and C/EBP-alpha are supposed to regulate SCD expression, expression levels of the two factors were not completely consistent with that of SCD.

Stearoyl-CoA desaturase mRNA expression during bovine adipocyte differentiation in primary culture derived from Japanese Black and Holstein cattle. Publishing Authors By Initials

H Ohsaki,T Sawa,S Sasazaki,K Kano,M Taniguchi,F Mukai,H Mannen,

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Stearoyl-CoA desaturase mRNA expression during bovine adipocyte differentiation in primary culture derived from Japanese Black and Holstein cattle. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Comparative biochemistry and physiology. Part A, M

VOLUME: 148

Page Numbers: 629-34

Journal Abbreviation: Comp. Biochem. Physiol., Part

ISSN: 1095-6433

DAY: 15

MONTH: 08

YEAR: 2007

Stearoyl-CoA desaturase mRNA expression during bovine adipocyte differentiation in primary culture derived from Japanese Black and Holstein cattle. Information

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LANGUAGE: eng

NlmUniqueID: 9806096

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Grant and Affiliation Information for Stearoyl-CoA desaturase mRNA expression during bovine adipocyte differentiation in primary culture derived from Japanese Black and Holstein cattle.

AFFILIATION: Laboratory of Animal Breeding and Genetics, Graduate School of Science and Technology, Kobe University, Kobe, Japan.

Country: United States

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MEDLINETA: Comp Biochem Physiol A Mol Int

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