Staufen1 is expressed in preimplantation mouse embryos and is required for embryonic stem cell differentiation.

Staufen1 is expressed in preimplantation mouse embryos and is required for embryonic stem cell differentiation. Research Abstract Details 

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Staufen1 is expressed in preimplantation mouse embryos and is required for embryonic stem cell differentiation. Abstract Text:

Pluripotent mouse embryonic stem (mES) cells derived from the blastocyst of the preimplantation embryo can be induced to differentiate in vitro along different cell lineages. However the molecular and cellular factors that signal and/or determine the expression of key genes, and the localisation of the encoded proteins, during the differentiation events are poorly understood. One common mechanism by which proteins can be targeted to specific regions of the cell is through the asymmetric localisation of mRNAs and Staufen, a double-stranded RNA binding protein, is known to play a direct role in mRNA transport and localisation. The aims of the present study were to describe the expression of Staufen in preimplantation embryos and mES cells and to use RNA interference (RNAi) to investigate the roles of Staufen1 in mES cell lineage differentiation. Western blotting and immunocytochemistry demonstrated that Staufen is present in the preimplantation mouse embryo, pluripotent mES cells and mES cells stimulated to differentiate into embryoid bodies, but the Staufen staining patterns did not support asymmetric distribution of the protein. Knockdown of Staufen1 gene expression in differentiating mES cells reduced the synthesis of lineage-specific markers including Brachyury, alpha-fetoprotein (AFP), PAX-6, and Vasa. There was however no significant change in either the gene expression of Nanog and Oct4, or in the synthesis of SSEA-1, all of which are key markers of pluripotency. These data indicate that inhibition of Staufen1 gene expression by RNAi affects an early step in mES cell differentiation and suggest a key role for Staufen in the cell lineage differentiation of mES cells.

Staufen1 is expressed in preimplantation mouse embryos and is required for embryonic stem cell differentiation. Publishing Authors By Initials

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Staufen1 is expressed in preimplantation mouse embryos and is required for embryonic stem cell differentiation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biochimica et biophysica acta

VOLUME: 1783

Page Numbers: 1935-42

Journal Abbreviation: Biochim. Biophys. Acta

ISSN: 0006-3002

DAY: 3

MONTH: 06

YEAR: 2008

Staufen1 is expressed in preimplantation mouse embryos and is required for embryonic stem cell differentiation. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Staufen1 is expressed in preimplantation mouse embryos and is required for embryonic stem cell differentiation.

AFFILIATION: Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Newcastle-upon-Tyne, NE2 4HH, UK.

Country: Netherlands

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MEDLINETA: Biochim Biophys Acta

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