STAT3 mediates bone marrow mesenchymal stem cell VEGF production.

STAT3 mediates bone marrow mesenchymal stem cell VEGF production. Research Abstract Details 

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STAT3 mediates bone marrow mesenchymal stem cell VEGF production. Abstract Text:

Meijing Wang,Wenjun Zhang,Paul Crisostomo,Troy Markel,Kirstan K Meldrum,Xin Y Fu,Daniel R Meldrum,

The mechanisms by which mesenchymal stem cells (MSCs) may protect native tissue are incompletely understood. Understanding the mechanisms by which these cells release factors such as vascular endothelial growth factor (VEGF), may lead to enhanced protection. We hypothesized that stress, in the form of hypoxia or TNF, activates MSCs to release VEGF by STAT3 and p38 MAPK dependent mechanisms. Mouse MSCs from wild type (WT) and STAT3 knockout mice (STAT3KO) were harvested and purified by a single-step method using adhesion. The release of VEGF was analyzed by using MSC conditioned media under hypoxia or TNF stimulation with or without p38 MAPK inhibition. Activation of STAT3 and p38 MAPK was determined by analysis of cell lysates. MSCs released VEGF under normoxia, which was associated with constitutive STAT3 activity. STAT3 deficiency resulted in decreased MSC production of VEGF. In response to hypoxia or TNF, MSCs produced more VEGF, which was correlated with hypoxia or TNF activated p38 MAPK and STAT3. The p38 MAPK inhibitor significantly decreased hypoxia-induced or TNF-stimulated VEGF production in WT. Additionally, STAT3 ablation neutralized hypoxia-induced MSC release of VEGF. No effect of p38 MAPK inhibitor alone was observed on MSC release of VEGF in WT. However, inhibition of p38 MAPK blocked release of VEGF in STAT3KO MSCs. MSCs are a potent source of VEGF, the production of which is mediated by STAT3 under normoxia partly; however, following hypoxia or TNF exposure, MSC release of VEGF is mediated by both STAT3 and p38 MAPK.

STAT3 mediates bone marrow mesenchymal stem cell VEGF production. Publishing Authors By Initials

M Wang,W Zhang,P Crisostomo,T Markel,KK Meldrum,XY Fu,DR Meldrum,

For similar peptides: intercellular signaling peptides and proteins: angiogenic proteins: vascular endothelial growth factors: vascular endothelial growth factor a research abstracts see: peptides: intercellular signaling peptides and proteins: angiogenic proteins: vascular endothelial growth factors: vascular endothelial growth factor a research

PUBMED ID PMID:

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STAT3 mediates bone marrow mesenchymal stem cell VEGF production. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of molecular and cellular cardiology

VOLUME: 42

Page Numbers: 1009-15

Journal Abbreviation: J. Mol. Cell. Cardiol.

ISSN: 0022-2828

DAY: 24

MONTH: 04

YEAR: 2007

STAT3 mediates bone marrow mesenchymal stem cell VEGF production. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 262322

STAT3 mediates bone marrow mesenchymal stem cell VEGF production. Keywords Mesh Terms:

KEYWORDS: Vascular Endothelial Growth Factor A

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: STAT3 mediates bone marrow mesenchymal stem cell VEGF production. Information

Substance Name: Vascular Endothelial Growth Factor A

Registry Number: 0

Grant and Affiliation Information for STAT3 mediates bone marrow mesenchymal stem cell VEGF production.

AFFILIATION: Department of Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA.

Country: England

AGENCY: United States NIGMS

GRANT: R01 GM 070628

ACRONYM: GM

MEDLINETA: J Mol Cell Cardiol

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