Staphylococcus aureus protein A induced inflammatory response in human corneal epithelial cells.

Staphylococcus aureus protein A induced inflammatory response in human corneal epithelial cells. Research Abstract Details 

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Staphylococcus aureus protein A induced inflammatory response in human corneal epithelial cells. Abstract Text:

Ashok Kumar,Alexander Mark Tassopoulos,Qiong Li,Fu-Shin X Yu,

In the present study, we examined the role of Staphylococcus aureus protein A (SpA) in inducing inflammatory response in human corneal epithelial cells (HCECs). Exposure of HCECs to SpA induces rapid NF-kappaB activation and secretion of proinflammatory cytokine/chemokines (TNF-alpha and IL-8) in both concentration and time-dependent manner. Challenge of HCECs with live SpA(-/-) mutant S. aureus strains resulted in significantly reduced production of the cytokines when compared to the wild-type S. aureus strain. SpA also elicited the activation of MAP Kinases P38, ERK, but not JNK, in HCECs. SpA-induced production of proinflammatory cytokine were completely blocked by the NF-kappaB and p38 inhibitors and partially inhibited by the Jnk inhibitor. Pretreatment with anti-TLR2 neutralizing antibody had no effect on SpA-induced inflammatory response in HCECs, suggesting that this response is independent of TLR2 signaling. Moreover, unlike TLR2 ligands, SpA failed to induce the expression of antimicrobial peptides (hBD2 and LL-37) in HCECs. These studies indicate that SpA is a S. aureus virulence factor that stimulates HCEC inflammatory response through a pathway distinct from TLR2 in HCECs.

Staphylococcus aureus protein A induced inflammatory response in human corneal epithelial cells. Publishing Authors By Initials

A Kumar,AM Tassopoulos,Q Li,FS Yu,

For similar peptides: intercellular signaling peptides and proteins: cytokines: monokines: tumor necrosis factor-alpha research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: monokines: tumor necrosis factor-alpha research

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MEDLINE DATE:

Staphylococcus aureus protein A induced inflammatory response in human corneal epithelial cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemical and biophysical research communication

VOLUME: 354

Page Numbers: 955-61

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 0006-291X

DAY: 23

MONTH: 01

YEAR: 2007

Staphylococcus aureus protein A induced inflammatory response in human corneal epithelial cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372516

Staphylococcus aureus protein A induced inflammatory response in human corneal epithelial cells. Keywords Mesh Terms:

KEYWORDS: Tumor Necrosis Factor-alpha

MESH TERMS: secretion

Chemical & Substance for Abstract: Staphylococcus aureus protein A induced inflammatory response in human corneal epithelial cells. Information

Substance Name: Mitogen-Activated Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Staphylococcus aureus protein A induced inflammatory response in human corneal epithelial cells.

AFFILIATION: The Kresge Eye Institute, Department of Ophthalmology, Wayne State University School of Medicine, Detroit, MI 48201, USA. akuma@med.wayne.edu

Country: United States

AGENCY: United States NEI

GRANT: R01 EY014080-04

ACRONYM: EY

MEDLINETA: Biochem Biophys Res Commun

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