Stabilization of proteins by recombinant human gelatins.

Stabilization of proteins by recombinant human gelatins. Research Abstract Details 

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Stabilization of proteins by recombinant human gelatins. Abstract Text:

Nagarajan Thyagarajapuram,David Olsen,C Russell Middaugh,Nagarajan Thyagarajapuram,David Olsen,C Russell Middaugh,Nagarajan Thyagarajapuram,David Olsen,C Russell Middaugh,

Porcine gelatins have been widely used as stabilizers of macromolecular based pharmaceuticals but the mechanism by which they stabilize has not been precisely established. Their variability and immunogenicity, however, make them less than ideal excipients. In this work, we take advantage of the availability of recombinant human gelatins (rhGs) to explore the mechanism by which they may stabilize proteins. Three model recombinant proteins, human serum albumin (HSA), bovine granulocyte colony stimulating factor (bGCSF), and human fibroblast growth factor-20 (FGF-20) that display a range of isoionic points have been selected for this study. The interaction of these model proteins with four different molecular weight rhGs and porcine gelatin was studied using a variety of biophysical techniques including fluorescence, CD and second derivative UV spectroscopy to monitor tertiary and secondary structure as a function of temperature. The 8.5, 25, and 100 kDa rhGs had the greatest effect on conformational and colloidal stability of HSA. The 8.5, 25, and 50 kDa rhGs also increased the T(0) of aggregation of bGCSF and FGF-20. Experiments to probe the mechanism of interaction of model proteins with rhGs suggest that the rhGs might interact with the partially unfolded states of target proteins through a combination of electrostatic and other intermolecular mechanisms to inhibit aggregation. (c) 2007 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 96: 3304-3315, 2007.

Stabilization of proteins by recombinant human gelatins. Publishing Authors By Initials

N Thyagarajapuram,D Olsen,CR Middaugh,N Thyagarajapuram,D Olsen,CR Middaugh,N Thyagarajapuram,D Olsen,CR Middaugh,

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Stabilization of proteins by recombinant human gelatins. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of pharmaceutical sciences

VOLUME: 96

Page Numbers: 3304-15

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ISSN: 0022-3549

DAY: 1

MONTH: Dec

YEAR: 2007

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LANGUAGE: eng

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AFFILIATION: Department of Pharmaceutical Chemistry, University of Kansas, 2030, Becker Drive, Lawrence, Kansas 66049.

Country: United States

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MEDLINETA: J Pharm Sci

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