Stability and transformation of the glucocorticoid receptor under acidic conditions.

Stability and transformation of the glucocorticoid receptor under acidic conditions. Research Abstract Details 

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Stability and transformation of the glucocorticoid receptor under acidic conditions. Abstract Text:

T Nemoto,Y Ohara-Nemoto,M Ota,

The [3H]triamcinolone acetonide ([3H]TA)-binding ability of the rat liver glucocorticoid receptor (GR) was investigated under acidic conditions, ranging from pH 2 to 7.3. Both in the presence and absence of 10 mM molybdate, the [3H]TA-binding ability decreased below pH 6.5 and was almost completely lost below pH 5, pH 5.9 +/- 0.1 giving 50% [3H]TA-binding. The binding ability was recovered when the pH of the cytosol was reversed to 7.3 or the precipitate obtained on acidification was dissolved in a buffer of pH 7.3. Moreover, in the absence of molybdate, the [3H]TA-GR complexes formed at pH 7.3 remained unchanged until pH 5. Then they decreased, pH 3.9 +/- 0.1 giving 50% binding, and completely disappeared at pH 3. [3H]TA-binding activity recovered from the precipitate also decreased in a similar pH region (a 50% decrease in binding being observed at pH 4.2 +/- 0.04). These results suggest that rat liver GR is rather resistant under acidic conditions and that it exists in a peculiar state below pH 5.9 to approximately 4 as to its ligand binding property: unoccupied GR has no [3H]TA-binding ability but [3H]TA-GR complexes once formed at neutral pH do not dissociate. [3H]TA-GR complexes recovered from the precipitate at pH 5 had a Stokes radius of 7.5 nm, little DNA-cellulose-binding ability and sedimented at 8.6S on glycerol gradient centrifugation, indicating that the receptor existed in a nontransformed state. In addition, both occupied and unoccupied GR were transformed at about pH 4, their being 50% transformation. This transformation was accompanied by irreversible denaturation of the receptor.(ABSTRACT TRUNCATED AT 250 WORDS)

Stability and transformation of the glucocorticoid receptor under acidic conditions. Publishing Authors By Initials

T Nemoto,Y Ohara-Nemoto,M Ota,

For similar polycyclic compounds: steroids: pregnanes: pregnadienes: triamcinolone research abstracts see: polycyclic compounds: steroids: pregnanes: pregnadienes: triamcinolone research

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Stability and transformation of the glucocorticoid receptor under acidic conditions. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 103

Page Numbers: 920-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1988

Stability and transformation of the glucocorticoid receptor under acidic conditions. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Stability and transformation of the glucocorticoid receptor under acidic conditions. Keywords Mesh Terms:

KEYWORDS: Triamcinolone

MESH TERMS: metabolism

Chemical & Substance for Abstract: Stability and transformation of the glucocorticoid receptor under acidic conditions. Information

Substance Name: Molybdenum

Registry Number: 7439-98-7

Grant and Affiliation Information for Stability and transformation of the glucocorticoid receptor under acidic conditions.

AFFILIATION: Department of Biochemistry, Iwate Medical University School of Dentistry.

Country: JAPAN

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MEDLINETA: J Biochem

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