Src and Pyk2 mediate angiotensin II effects in cultured rat astrocytes.

Src and Pyk2 mediate angiotensin II effects in cultured rat astrocytes. Research Abstract Details 

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Src and Pyk2 mediate angiotensin II effects in cultured rat astrocytes. Abstract Text:

Michelle A Clark,Noelvy Gonzalez,

Angiotensin II (Ang II)-induced proliferation of rat astrocytes is mediated by multiple signaling pathways. In the present study, we investigated the role of non-receptor tyrosine kinases on Ang II-signaling and proliferation of astrocytes cultured from neonatal rat pups. Ang II stimulated astrocyte growth, ERK1/2 phosphorylation and the phosphorylation of Src and proline-rich tyrosine kinase-2 (Pyk2), in astrocytes obtained from brainstem and cerebellum. Pretreatment with 10 microM PP2, a selective Src inhibitor, inhibited Ang II stimulated ERK1/2 phosphorylation by 59% to 91% both in brainstem and cerebellum astrocytes. PP2 also inhibited Ang II induction of brainstem (76% inhibition) and cerebellar (64% inhibition) astrocyte growth. Similarly, pretreatment with 25 microM dantrolene, the Pyk2 inhibitor, attenuated ERK1/2 activity in brainstem (62% inhibition) and in cerebellum astrocytes (44% inhibition). Interestingly, inhibition of Pyk2 inhibited Ang II-induced Src activation suggesting that these two non-receptor tyrosine kinases may be acting in concert to mediate Ang II effects in astrocytes. In summary, we found that Ang II stimulates the non-receptor tyrosine kinases Src and Pyk2 which mediate Ang II-induced ERK1/2 activation leading to stimulation of astrocyte growth. In addition, these two tyrosine kinases may be interacting to regulate effects of the peptide in these cells.

Src and Pyk2 mediate angiotensin II effects in cultured rat astrocytes. Publishing Authors By Initials

MA Clark,N Gonzalez,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, sprague-dawley research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, sprague-dawley research

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Src and Pyk2 mediate angiotensin II effects in cultured rat astrocytes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Regulatory peptides

VOLUME: 143

Page Numbers: 47-55

Journal Abbreviation: Regul. Pept.

ISSN: 0167-0115

DAY: 28

MONTH: 02

YEAR: 2007

Src and Pyk2 mediate angiotensin II effects in cultured rat astrocytes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8100479

Src and Pyk2 mediate angiotensin II effects in cultured rat astrocytes. Keywords Mesh Terms:

KEYWORDS: Rats, Sprague-Dawley

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Src and Pyk2 mediate angiotensin II effects in cultured rat astrocytes. Information

Substance Name: Mitogen-Activated Protein Kinase 1

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Src and Pyk2 mediate angiotensin II effects in cultured rat astrocytes.

AFFILIATION: Department of Pharmaceutical and Administrative Sciences, College of Pharmacy, Nova Southeastern University, 3200 South University Drive, Fort Lauderdale, FL 33328, USA. miclark@nova.edu

Country: Netherlands

AGENCY: United States NHLBI

GRANT: HL-077199

ACRONYM: HL

MEDLINETA: Regul Pept

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