Spleen cell phosphorylation of salt soluble nuclear protein from isoproterenol treated and sorted nuclei.

Spleen cell phosphorylation of salt soluble nuclear protein from isoproterenol treated and sorted nuclei. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Spleen cell phosphorylation of salt soluble nuclear protein from isoproterenol treated and sorted nuclei. Abstract Text:

J L Pipkin,J F Anson,W G Hinson,H Schol,D M Sheehan,

The effect of isoproterenol (IPR) on phosphorylation of acidic nuclear proteins was investigated by two-dimensional gel autoradiography. Mouse spleen cells stimulated to divide by the mitogen concanavalin A (Con A) were separated according to cell cycle stage by flow microfluorometric technique. Exposure of cells for 48 h to 4 micrograms IPR/ml culture medium produced no significant change in the proportion of S and G2 phase cells, while a cumulative dose of 8 micrograms IPR/ml caused a significant repression in DNA synthesis and a reduction in the number of nuclei in G2 + M phase. Four micrograms IPR/ml stimulated the greatest amount of G0 + G1 phosphorylation of nuclear protein. Several proteins from G0 + G1 and S nuclei incorporated 32P after Con A + IPR administration, and one protein from S phase nuclei revealed intensified labeling at the 8 micrograms cumulative IPR dose but not at the 4 micrograms dose. The isolated proteins (W, X, Y, and Z) were reassociated with homologous DNA, centrifuged in a sucrose gradient and shown to co-sediment with DNA. S phase nuclear protein X-S, which was found to be a mixture of proteins (X0 and X1), was the only exception. One component of X-S, X0 bound to DNA, while component X1 failed to bind. Chymotryptic and V8 protease digests of all isolated proteins were made and analyzed by autoradiography. Proteins X0 and X1, recovered from the sucrose gradient, possessed dissimilar fragment patterns. It is concluded that protein X-S is composed of two proteins (X0 and X1), one of which (X1) appears during S phase during the 8 micrograms IPR induced nuclear repression.

Spleen cell phosphorylation of salt soluble nuclear protein from isoproterenol treated and sorted nuclei. Publishing Authors By Initials

JL Pipkin,JF Anson,WG Hinson,H Schol,DM Sheehan,

For similar tissues: lymphoid tissue: spleen research abstracts see: tissues: lymphoid tissue: spleen research

PUBMED ID PMID:

MEDLINE DATE:

Spleen cell phosphorylation of salt soluble nuclear protein from isoproterenol treated and sorted nuclei. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 95

Page Numbers: 323-33

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1984

Spleen cell phosphorylation of salt soluble nuclear protein from isoproterenol treated and sorted nuclei. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Spleen cell phosphorylation of salt soluble nuclear protein from isoproterenol treated and sorted nuclei. Keywords Mesh Terms:

KEYWORDS: Spleen

MESH TERMS: metabolism

Chemical & Substance for Abstract: Spleen cell phosphorylation of salt soluble nuclear protein from isoproterenol treated and sorted nuclei. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Spleen cell phosphorylation of salt soluble nuclear protein from isoproterenol treated and sorted nuclei.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Spleen cell phosphorylation of salt soluble nuclear protein from isoproterenol treated and sorted nuclei Related Publications

• A comparison of diabetic foot ulcer outcomes using negative pressure wound therapy versus historical standard of care.
• A new vision for an aging population. The church can play a major role.
• A study of the intracellular and secreted forms of the MUC2 mucin from the PC/AA intestinal cell line.
• Alcohol-based instant hand sanitizer use in military settings: a prospective cohort study of Army basic trainees.
• Arthroplasty of the knee.
• Characterization of mucins from cultured normal human tracheobronchial epithelial cells.
• Concentrated solutions of salivary MUC5B mucin do not replicate the gel-forming properties of saliva.
• Cumulative asbestos exposure for US automobile mechanics involved in brake repair (circa 1950s-2000).
• Current concepts in management of meningiomas and schwannomas.
• D-phenylglycinol-derived non-covalent factor Xa inhibitors: effect of non-peptidic S4 linkage elements on affinity and anticoagulant activity.
• Evaluation of a Repeat Sprint Test for Australian Football: Relationship to Speed and Endurance: 1443: Board #206 May 30 9:30 AM - 11:00 AM.
• Foam cell plaques in intima of myometrial arteries; a presumptive sign of previous irradiation.
• Identification of a nonmucin glycoprotein (gp-340) from a purified respiratory mucin preparation: evidence for an association involving the MUC5B mucin.
• Identification of two glycoforms of the MUC5B mucin in human respiratory mucus. Evidence for a cysteine-rich sequence repeated within the molecule.
• Optic atrophy with altitudinal hemianopia in neurofibromatosis.
• Paroxysmal haemoglobinuria with a report on three cases.
• Pediatric advocacy.
• Preliminary study pointing out a significant alteration in the biochemical composition of MUC2 in colorectal mucinous carcinoma.
• Proteomic identification of tyrosine nitration targets in kidney of spontaneously hypertensive rats.
• Redox proteomics in the mussel, Mytilus edulis.
• Retained placenta and postpartum haemorrhage.
• Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product.
• Simulation tests to assess occupational exposure to airborne asbestos from asphalt-based roofing products.
• Spatial analysis of colorectal cancer incidence and proportion of late-stage in Massachusetts residents: 1995-1998.
• Spleen cell phosphorylation of salt soluble nuclear protein from isoproterenol treated and sorted nuclei.
• The efficacy of vaginal clindamycin for the treatment of abnormal genital tract flora in pregnancy.
• The heparin-binding exosite is critical to allosteric activation of factor IXa in the intrinsic tenase complex: the role of arginine 165 and factor X.
• The potential of proteomics for providing new insights into environmental impacts on human health.
• The silence of suffering: breaking the sound barriers.
• Three-dimensional object representation and invariant recognition using continuous distance transform neural networks.