Spindle oscillations during asymmetric cell division require a threshold number of active cortical force generators.

Spindle oscillations during asymmetric cell division require a threshold number of active cortical force generators. Research Abstract Details 

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Spindle oscillations during asymmetric cell division require a threshold number of active cortical force generators. Abstract Text:

BACKGROUND: Asymmetric division of the C. elegans zygote is due to the posterior-directed movement of the mitotic spindle during metaphase and anaphase. During this movement along the anterior-posterior axis, the spindle oscillates transversely. These motions are thought to be driven by a force-generating complex-possibly containing the motor protein cytoplasmic dynein-that is located at the cell cortex and pulls on microtubules growing out from the spindle poles. A theoretical analysis indicates that the oscillations might arise from mechanical coordination of the force-generating motors, and this coordination is mediated by the load dependence of the motors' detachment from the microtubules. The model predicts that the motor activity must exceed a threshold for oscillations to occur. RESULTS: We have tested the existence of a threshold by using RNA interference to gradually reduce the levels of dynein light intermediate chain as well as GPR-1 and GPR-2 that are involved in the G protein-mediated regulation of the force generators. We found an abrupt cessation of oscillations as expected if the motor activity dropped below a threshold. Furthermore, we can account for the complex choreography of the mitotic spindle-the precise temporal coordination of the buildup and die-down of the transverse oscillations with the posterior displacement-by a gradual increase in the processivity of a single type of motor machinery during metaphase and anaphase. CONCLUSIONS: The agreement between our results and modeling suggests that the force generators themselves have the intrinsic capability of generating oscillations when opposing forces exceed a threshold.

Spindle oscillations during asymmetric cell division require a threshold number of active cortical force generators. Publishing Authors By Initials

For similar genetic processes: gene expression regulation: epigenesis, genetic: gene silencing: rna interference research abstracts see: genetic processes: gene expression regulation: epigenesis, genetic: gene silencing: rna interference research

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Spindle oscillations during asymmetric cell division require a threshold number of active cortical force generators. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Current biology : CB

VOLUME: 16

Page Numbers: 2111-22

Journal Abbreviation: Curr. Biol.

ISSN: 0960-9822

DAY: 7

MONTH: Nov

YEAR: 2006

Spindle oscillations during asymmetric cell division require a threshold number of active cortical force generators. Information

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LANGUAGE: eng

NlmUniqueID: 9107782

Spindle oscillations during asymmetric cell division require a threshold number of active cortical force generators. Keywords Mesh Terms:

KEYWORDS: RNA Interference

MESH TERMS: physiology

Chemical & Substance for Abstract: Spindle oscillations during asymmetric cell division require a threshold number of active cortical force generators. Information

Substance Name: Dynein ATPase

Registry Number: EC 3.6.4.2

Grant and Affiliation Information for Spindle oscillations during asymmetric cell division require a threshold number of active cortical force generators.

AFFILIATION: Max-Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.

Country: England

AGENCY: United States NIAMS

GRANT: AR40593

ACRONYM: AR

MEDLINETA: Curr Biol

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