Spectrum and range of oxidative stress responses of human lens epithelial cells to H2O2 insult.

Spectrum and range of oxidative stress responses of human lens epithelial cells to H2O2 insult. Research Abstract Details 

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Spectrum and range of oxidative stress responses of human lens epithelial cells to H2O2 insult. Abstract Text:

Sumanta Goswami,Nancy L Sheets,Jiri Zavadil,Bharesh K Chauhan,Erwin P Bottinger,Venkat N Reddy,Marc Kantorow,Ales Cvekl,

PURPOSE: Oxidative stress (OS) is believed to be a major contributor to age-related cataract and other age-related diseases. METHODS: cDNA microarrays were used to identify the spectrum and range of genes with transcript levels that are altered in response to acute H(2)O(2)-induced OS in human lens epithelial (HLE) cells. HLE cells were treated with 50 microM H(2)O(2) for 1 hour in the absence of serum, followed by a return to complete medium. RNAs were prepared from treated and untreated cells at 0, 1, 2, and 8 hours after H(2)O(2) treatment. RESULTS: The data showed 1171 genes that were significantly up- and downregulated in response to H(2)O(2) treatment. Several functional subcategories of genes were identified, including those encoding DNA repair proteins, antioxidant defense enzymes, molecular chaperones, protein biosynthesis enzymes, and trafficking and degradation proteins. Differential expression of selected genes was confirmed at the level of RNA and/or protein. Many of the identified genes (e.g., glutathione S-transferase [MGST2], thioredoxin reductase beta, and peroxiredoxin 2) have been identified as participants in OS responses in the lens and other systems. Some genes induced by OS in the current study (e.g., oxygen regulated protein [ORP150] and heat shock protein [HSP40]) are better known to respond to other forms of stress. Two genes (receptor tyrosine kinase [AXL/ARK] and protein phosphatase 2A) are known to be differentially expressed in cataract. Most of the genes point to a novel pathways associated with OS. CONCLUSIONS: The present data provide a global perspective on those genes that respond to acute OS, point to novel genes and pathways associated with OS, and set the groundwork for understanding the functions of OS-related genes in lens protection and disease.

Spectrum and range of oxidative stress responses of human lens epithelial cells to H2O2 insult. Publishing Authors By Initials

S Goswami,NL Sheets,J Zavadil,BK Chauhan,EP Bottinger,VN Reddy,M Kantorow,A Cvekl,

For similar genetic processes: gene expression regulation: up-regulation research abstracts see: genetic processes: gene expression regulation: up-regulation research

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Spectrum and range of oxidative stress responses of human lens epithelial cells to H2O2 insult. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Investigative ophthalmology & visual science

VOLUME: 44

Page Numbers: 2084-93

Journal Abbreviation: Invest. Ophthalmol. Vis. Sci.

ISSN: 0146-0404

DAY: 26

MONTH: May

YEAR: 2003

Spectrum and range of oxidative stress responses of human lens epithelial cells to H2O2 insult. Information

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LANGUAGE: eng

NlmUniqueID: 7703701

Spectrum and range of oxidative stress responses of human lens epithelial cells to H2O2 insult. Keywords Mesh Terms:

KEYWORDS: Up-Regulation

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Spectrum and range of oxidative stress responses of human lens epithelial cells to H2O2 insult. Information

Substance Name: Hydrogen Peroxide

Registry Number: 7722-84-1

Grant and Affiliation Information for Spectrum and range of oxidative stress responses of human lens epithelial cells to H2O2 insult.

AFFILIATION: Department of Ophthalmology and Visual Sciences, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

Country: United States

AGENCY: United States NEI

GRANT: R01 EY013022-02

ACRONYM: EY

MEDLINETA: Invest Ophthalmol Vis Sci

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