SPECT imaging of myocardial infarction using (99m)Tc-labeled C2A domain of synaptotagmin I in a porcine ischemia-reperfusion model.

SPECT imaging of myocardial infarction using (99m)Tc-labeled C2A domain of synaptotagmin I in a porcine ischemia-reperfusion model. Research Abstract Details 

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SPECT imaging of myocardial infarction using (99m)Tc-labeled C2A domain of synaptotagmin I in a porcine ischemia-reperfusion model. Abstract Text:

Wei Fang,Feng Wang,Shundong Ji,Xiaoguang Zhu,Heidi T Meier,Robert S Hellman,Kevin M Brindle,Bazbek Davletov,Ming Zhao,Wei Fang,Feng Wang,Shundong Ji,Xiaoguang Zhu,Heidi T Meier,Robert S Hellman,Kevin M Brindle,Bazbek Davletov,Ming Zhao,

INTRODUCTION: The C2A domain of synaptotagmin I recognizes necrotic and apoptotic cells by binding to exposed anionic phospholipids. The goal is to explore the potential imaging utility of (99m)Tc-labeled C2A in the detection of acute cardiac cell death in a porcine model that resembles human cardiovascular physiology. METHODS: Ischemia (20-25 min) was induced in pigs (M/F, 20-25 kg) using balloon angioplasty. (99m)Tc-C2A-GST (n=7) or (99m)Tc-BSA (n=2) was injected intravenously 1-2 h after reperfusion. Noninfarct animals were injected with (99m)Tc-C2A-GST (n=4). SPECT images were acquired at 3 and 6 h postinjection. Cardiac tissues were analyzed to confirm the presence of cell death. RESULTS: Focal uptake was detected in five out of seven subjects at 3 h and in all infarct subjects at 6 h postinjection but not in infarct animals injected with (99m)Tc-BSA or in noninfarct animals with (99m)Tc-C2A-GST. Gamma counting of infarct versus normal myocardium yielded a 10.2+/-5.7-fold elevation in absolute radioactivity, with histologically confirmed infarction. CONCLUSIONS: We present data on imaging myocardial cell death in the acute phase of infarction in pigs. C2A holds promise and warrants further development as an infarct-avid molecular probe.

SPECT imaging of myocardial infarction using (99m)Tc-labeled C2A domain of synaptotagmin I in a porcine ischemia-reperfusion model. Publishing Authors By Initials

W Fang,F Wang,S Ji,X Zhu,HT Meier,RS Hellman,KM Brindle,B Davletov,M Zhao,W Fang,F Wang,S Ji,X Zhu,HT Meier,RS Hellman,KM Brindle,B Davletov,M Zhao,

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SPECT imaging of myocardial infarction using (99m)Tc-labeled C2A domain of synaptotagmin I in a porcine ischemia-reperfusion model. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Nuclear medicine and biology

VOLUME: 34

Page Numbers: 917-23

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ISSN: 0969-8051

DAY: 4

MONTH: 09

YEAR: 2007

SPECT imaging of myocardial infarction using (99m)Tc-labeled C2A domain of synaptotagmin I in a porcine ischemia-reperfusion model. Information

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LANGUAGE: eng

NlmUniqueID: 9304420

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AFFILIATION: Department of Nuclear Medicine, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences, Beijing 100037, China.

Country: England

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MEDLINETA: Nucl Med Biol

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