Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the Foxn1 locus.

Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the Foxn1 locus. Research Abstract Details 

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Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the Foxn1 locus. Abstract Text:

Julie Gordon,Shiyun Xiao,Bernard Hughes,Dong-ming Su,Samuel P Navarre,Brian G Condie,Nancy R Manley,

BACKGROUND: Thymic epithelial cells (TECs) promote thymocyte maturation and are required for the early stages of thymocyte development and for positive selection. However, investigation of the mechanisms by which TECs perform these functions has been inhibited by the lack of genetic tools. Since the Foxn1 gene is expressed in all presumptive TECs from the early stages of thymus organogenesis and broadly in the adult thymus, it is an ideal locus for driving gene expression in differentiating and mature TECs. RESULTS: We generated two knock-in alleles of Foxn1 by inserting IRES-Cre or IRES-lacZ cassettes into the 3' UTR of the Foxn1 locus. We simultaneously electroporated the two targeting vectors to generate the two independent alleles in the same experiment, demonstrating the feasibility of multiplex gene targeting at this locus. Our analysis shows that the knockin alleles drive expression of Cre or lacZ in all TECs in the fetal thymus. Furthermore, the knockin alleles express Cre or lacZ in a Foxn1-like pattern without disrupting Foxn1 function as determined by phenotype analysis of Foxn1 knockin/Foxn1 null compound heterozygotes. CONCLUSION: These data show that multiplex gene targeting into the 3' UTR of the Foxn1 locus is an efficient method to express any gene of interest in TECs from the earliest stage of thymus organogenesis. The resulting alleles will make possible new molecular and genetic studies of TEC differentiation and function. We also discuss evidence indicating that gene targeting into the 3' UTR is a technique that may be broadly applicable for the generation of genetically neutral driver strains.

Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the Foxn1 locus. Publishing Authors By Initials

J Gordon,S Xiao,B Hughes,DM Su,SP Navarre,BG Condie,NR Manley,

For similar tissues: lymphoid tissue: thymus gland research abstracts see: tissues: lymphoid tissue: thymus gland research

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Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the Foxn1 locus. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: BMC developmental biology

VOLUME: 7

Page Numbers: 69

Journal Abbreviation: BMC Dev. Biol.

ISSN: 1471-213X

DAY: 18

MONTH: 06

YEAR: 2007

Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the Foxn1 locus. Information

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LANGUAGE: eng

NlmUniqueID: 100966973

Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the Foxn1 locus. Keywords Mesh Terms:

KEYWORDS: Thymus Gland

MESH TERMS: growth & development

Chemical & Substance for Abstract: Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the Foxn1 locus. Information

Substance Name: Integrases

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the Foxn1 locus.

AFFILIATION: Department of Genetics, University of Georgia, Athens, GA 30602, USA. jgordon@uga.edu <jgordon@uga.edu>

Country: England

AGENCY: United States NICHD

GRANT: HD043479

ACRONYM: HD

MEDLINETA: BMC Dev Biol

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