Specific detection of N-acetylglucosamine-containing oligosaccharide chains on ovine submaxillary asialomucin.

Specific detection of N-acetylglucosamine-containing oligosaccharide chains on ovine submaxillary asialomucin. Research Abstract Details 

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Specific detection of N-acetylglucosamine-containing oligosaccharide chains on ovine submaxillary asialomucin. Abstract Text:

D H Van den Eijnden,W E Schiphorst,E G Berger,

Human milk beta-N-acetylglucosaminide beta 1 leads to 4-galactosyltransferase (EC 2.4.1.38) was used to galactosylate ovine submaxillary asialomucin to saturation. The major [14C]galactosylated product chain was obtained as a reduced oligosaccharide by beta-elimination under reducing conditions. Analysis by Bio-Gel filtration and gas-liquid chromatography indicated that this compound was a tetrasaccharide composed of galactose, N-acetylglucosamine and reduced N-acetylgalactosamine in a molar ratio of 2:0.9:0.8. Periodate oxidation studies before and after mild acid hydrolysis in addition to thin-layer chromatography revealed that the most probable structure of the tetrasaccharide is Gal beta 1 leads to 3([14C]Gal beta 1 leads to 4GlcNAc beta 1 leads to 6)GalNAcol. Thus it appears that Gal beta 1 leads to 3(GlcNAc beta 1 leads to 6)GalNAc units occur as minor chains on the asialomucin. The potential interference of these chains in the assay of alpha-N-acetylgalactosaminylprotein beta 1 leads to 3-galactosyltransferase activity using ovine submaxillary asialomucin as an acceptor can be counteracted by the addition of N-acetylglucosamine.

Specific detection of N-acetylglucosamine-containing oligosaccharide chains on ovine submaxillary asialomucin. Publishing Authors By Initials

DH Van den Eijnden,WE Schiphorst,EG Berger,

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Specific detection of N-acetylglucosamine-containing oligosaccharide chains on ovine submaxillary asialomucin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochimica et biophysica acta

VOLUME: 755

Page Numbers: 32-9

Journal Abbreviation: Biochim. Biophys. Acta

ISSN: 0006-3002

DAY: 4

MONTH: Jan

YEAR: 1983

Specific detection of N-acetylglucosamine-containing oligosaccharide chains on ovine submaxillary asialomucin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 217513

Specific detection of N-acetylglucosamine-containing oligosaccharide chains on ovine submaxillary asialomucin. Keywords Mesh Terms:

KEYWORDS: beta-N-Acetylglucosaminylglycopeptide be

MESH TERMS: metabolism

Chemical & Substance for Abstract: Specific detection of N-acetylglucosamine-containing oligosaccharide chains on ovine submaxillary asialomucin. Information

Substance Name: beta-N-Acetylglucosaminylglycopeptide be

Registry Number: EC 2.4.1.38

Grant and Affiliation Information for Specific detection of N-acetylglucosamine-containing oligosaccharide chains on ovine submaxillary asialomucin.

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Country: NETHERLANDS

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MEDLINETA: Biochim Biophys Acta

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