Spatial photorelease of oligonucleotides, using a safety-catch photolabile linker.

Spatial photorelease of oligonucleotides, using a safety-catch photolabile linker. Research Abstract Details 

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Spatial photorelease of oligonucleotides, using a safety-catch photolabile linker. Abstract Text:

Shane T Flickinger,Madhusudan Patel,Brock F Binkowski,Aaron M Lowe,Mo-Huang Li,Changhan Kim,Franco Cerrina,Peter J Belshaw,

[reaction: see text] We report the development of a safety-catch photolabile linker that allows the light-directed synthesis and spatially selective photorelease of oligonucleotides from microarrays. The linker remains stable to light during DNA synthesis, and is activated for photorelease after acidic hydrolysis. We demonstrate that the photoreleased oligonucleotides can be amplified by PCR to produce double stranded DNA. The advantages offered by this linker could aid the development of an automated gene synthesis platform.

Spatial photorelease of oligonucleotides, using a safety-catch photolabile linker. Publishing Authors By Initials

ST Flickinger,M Patel,BF Binkowski,AM Lowe,MH Li,C Kim,F Cerrina,PJ Belshaw,

For similar natural sciences: chemistry: chemistry, physical: photochemistry: photolysis research abstracts see: natural sciences: chemistry: chemistry, physical: photochemistry: photolysis research

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Spatial photorelease of oligonucleotides, using a safety-catch photolabile linker. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Organic letters

VOLUME: 8

Page Numbers: 2357-60

Journal Abbreviation: Org. Lett.

ISSN: 1523-7060

DAY: 25

MONTH: May

YEAR: 2006

Spatial photorelease of oligonucleotides, using a safety-catch photolabile linker. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100890393

Spatial photorelease of oligonucleotides, using a safety-catch photolabile linker. Keywords Mesh Terms:

KEYWORDS: Photolysis

MESH TERMS: chemistry

Chemical & Substance for Abstract: Spatial photorelease of oligonucleotides, using a safety-catch photolabile linker. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Spatial photorelease of oligonucleotides, using a safety-catch photolabile linker.

AFFILIATION: The Department of Chemistry, University of Wisconsin-Madison, 53706, USA.

Country: United States

AGENCY: United States NCRR

GRANT: RR08389

ACRONYM: RR

MEDLINETA: Org Lett

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