SPARC regulates processing of procollagen I and collagen fibrillogenesis in dermal fibroblasts.

SPARC regulates processing of procollagen I and collagen fibrillogenesis in dermal fibroblasts. Research Abstract Details 

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SPARC regulates processing of procollagen I and collagen fibrillogenesis in dermal fibroblasts. Abstract Text:

Tyler J Rentz,Felicitta Poobalarahi,Paul Bornstein,E Helene Sage,Amy D Bradshaw,

A characterization of the factors that control collagen fibril formation is critical for an understanding of tissue organization and the mechanisms that lead to fibrosis. SPARC (secreted protein acidic and rich in cysteine) is a counter-adhesive protein that binds collagens. Herein we show that collagen fibrils in SPARC-null skin from mice 1 month of age were inefficient in fibril aggregation and accumulated in the diameter range of 60-70 nm, a proposed intermediate in collagen fibril growth. In vitro, procollagen I produced by SPARC-null dermal fibroblasts demonstrated an initial preferential association with cell layers, in comparison to that produced by wild-type fibroblasts. However, the collagen I produced by SPARC-null cells was not efficiently incorporated into detergent-insoluble fractions. Coincident with an initial increase in cell association, greater amounts of total collagen I were present as processed forms in SPARC-null versus wild-type cells. Addition of recombinant SPARC reversed collagen I association with cell layers and decreased the processing of procollagen I in SPARC-null cells. Although collagen fibers formed on the surface of SPARC-null fibroblasts earlier than those on wild-type cells, fibers on SPARC-null fibroblasts did not persist. We conclude that SPARC mediates the association of procollagen I with cells, as well as its processing and incorporation into the extracellular matrix.

SPARC regulates processing of procollagen I and collagen fibrillogenesis in dermal fibroblasts. Publishing Authors By Initials

TJ Rentz,F Poobalarahi,P Bornstein,EH Sage,AD Bradshaw,

For similar digestive, oral, and skin physiology: skin physiology research abstracts see: digestive, oral, and skin physiology: skin physiology research

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SPARC regulates processing of procollagen I and collagen fibrillogenesis in dermal fibroblasts. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 22062-71

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 23

MONTH: 05

YEAR: 2007

SPARC regulates processing of procollagen I and collagen fibrillogenesis in dermal fibroblasts. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

SPARC regulates processing of procollagen I and collagen fibrillogenesis in dermal fibroblasts. Keywords Mesh Terms:

KEYWORDS: Skin Physiology

MESH TERMS: metabolism

Chemical & Substance for Abstract: SPARC regulates processing of procollagen I and collagen fibrillogenesis in dermal fibroblasts. Information

Substance Name: Collagen

Registry Number: 9007-34-5

Grant and Affiliation Information for SPARC regulates processing of procollagen I and collagen fibrillogenesis in dermal fibroblasts.

AFFILIATION: Department of Medicine, Medical University of South Carolina, Charleston, SC 29412, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: K01 GM40711

ACRONYM: GM

MEDLINETA: J Biol Chem

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