Sp1 and Sp3 mediate NHE2 gene transcription in the intestinal epithelial cells.

Sp1 and Sp3 mediate NHE2 gene transcription in the intestinal epithelial cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Sp1 and Sp3 mediate NHE2 gene transcription in the intestinal epithelial cells. Abstract Text:

Ping Hua,Hua Xu,Jennifer K Uno,Maciej A Lipko,Jiali Dong,Pawel R Kiela,Fayez K Ghishan,

Our previous studies have identified a minimal Sp1-driven promoter region (nt -36/+116) directing NHE2 expression in mouse renal epithelial cells. However, this minimal promoter region was not sufficient to support active transcription of NHE2 gene in the intestinal epithelial cells, suggesting the need for additional upstream regulatory elements. In the present study, we used nontransformed rat intestinal epithelial (RIE) cells as a model to identify the minimal promoter region and transcription factors necessary for the basal transcription of rat NHE2 gene in the intestinal epithelial cells. We identified a region within the rat NHE2 gene promoter located within nt -67/-43 upstream of transcription initiation site as indispensable for the promoter function in intestinal epithelial cells. Mutations at nt -56/-51 not only abolished the DNA-protein interaction in this region, but also completely abolished NHE2 gene promoter activity in RIE cells. Supershift assays revealed that Sp1 and Sp3 interact with this promoter region, but, contrary to the minimal promoter indispensable for renal expression of NHE2, both transcription factors expressed individually in Drosophila SL2 cells activated rat NHE2 gene promoter. Moreover, Sp1 was a weaker transactivator and when coexpressed in SL2 cells it reduced Sp3-mediated NHE2 basal promoter activity. Furthermore, DNase I footprinting confirmed that nt -58/-51 is protected by nuclear protein from RIE cells. We conclude that the mechanism of basal control of rat NHE2 gene promoter activity is different in the renal and intestinal epithelium, with Sp3 being the major transcriptional activator of NHE2 gene transcription in the intestinal epithelial cells.

Sp1 and Sp3 mediate NHE2 gene transcription in the intestinal epithelial cells. Publishing Authors By Initials

P Hua,H Xu,JK Uno,MA Lipko,J Dong,PR Kiela,FK Ghishan,

For similar proteins: dna-binding proteins: kruppel-like transcription factors: sp transcription factors: sp3 transcription factor research abstracts see: proteins: dna-binding proteins: kruppel-like transcription factors: sp transcription factors: sp3 transcription factor research

PUBMED ID PMID:

MEDLINE DATE:

Sp1 and Sp3 mediate NHE2 gene transcription in the intestinal epithelial cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of physiology. Gastrointestinal a

VOLUME: 293

Page Numbers: G146-53

Journal Abbreviation: Am. J. Physiol. Gastrointest.

ISSN: 0193-1857

DAY: 22

MONTH: 03

YEAR: 2007

Sp1 and Sp3 mediate NHE2 gene transcription in the intestinal epithelial cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901227

Sp1 and Sp3 mediate NHE2 gene transcription in the intestinal epithelial cells. Keywords Mesh Terms:

KEYWORDS: Sp3 Transcription Factor

MESH TERMS: physiology

Chemical & Substance for Abstract: Sp1 and Sp3 mediate NHE2 gene transcription in the intestinal epithelial cells. Information

Substance Name: Sp3 Transcription Factor

Registry Number: 148710-94-5

Grant and Affiliation Information for Sp1 and Sp3 mediate NHE2 gene transcription in the intestinal epithelial cells.

AFFILIATION: Department of Pediatrics, Steele Memorial Children's Research Center, 1501 N. Campbell Ave., Tucson, AZ 85724, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: 5R01 DK 41274

ACRONYM: DK

MEDLINETA: Am J Physiol Gastrointest Live

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Sp1 and Sp3 mediate NHE2 gene transcription in the intestinal epithelial cells Related Publications

• Apical NA+/H+ exchangers in the mammalian gastrointestinal tract.
• Colonic gene expression profile in NHE3-deficient mice: evidence for spontaneous distal colitis.
• Gastrointestinal Distribution and Kinetic Characterization of the Sodium-Hydrogen Exchanger Isoform 8 (NHE8).
• Molecular mechanism of rat NHE3 gene promoter regulation by sodium butyrate.
• Sp1 and Sp3 mediate NHE2 gene transcription in the intestinal epithelial cells.
• Sp1 is required for glucose-induced transcriptional regulation of mouse vesicular glutamate transporter 2 gene.