SP-A permeabilizes lipopolysaccharide membranes by forming protein aggregates that extract lipids from the membrane.

SP-A permeabilizes lipopolysaccharide membranes by forming protein aggregates that extract lipids from the membrane. Research Abstract Details 

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SP-A permeabilizes lipopolysaccharide membranes by forming protein aggregates that extract lipids from the membrane. Abstract Text:

Surfactant protein A (SP-A) is known to cause bacterial permeabilization. The aim of this work was to gain insight into the mechanism by which SP-A induces permeabilization of rough lipopolysaccharide (Re-LPS) membranes. In the presence of calcium, large interconnected aggregates of fluorescently labeled TR-SP-A were observed on the surface of Re-LPS films by epifluorescence microscopy. Using Re-LPS monolayer relaxation experiments at constant surface pressure, we demonstrated that SP-A induced Re-LPS molecular loss by promoting the formation of three-dimensional lipid-protein aggregates in Re-LPS membranes. This resulted in decreased van der Waals interactions between Re-LPS acyl chains, as determined by differential scanning calorimetry, which rendered the membrane leaky. We also showed that the coexistence of gel and fluid lipid phases within the Re-LPS membrane conferred susceptibility to SP-A-mediated permeabilization. Taken together, our results seem to indicate that the calcium-dependent permeabilization of Re-LPS membranes by SP-A is related to the extraction of LPS molecules from the membrane due to the formation of calcium-mediated protein aggregates that contain LPS.

SP-A permeabilizes lipopolysaccharide membranes by forming protein aggregates that extract lipids from the membrane. Publishing Authors By Initials

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SP-A permeabilizes lipopolysaccharide membranes by forming protein aggregates that extract lipids from the membrane. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biophysical journal

VOLUME: 95

Page Numbers: 3287-94

Journal Abbreviation: Biophys. J.

ISSN: 1542-0086

DAY: 3

MONTH: 07

YEAR: 2008

SP-A permeabilizes lipopolysaccharide membranes by forming protein aggregates that extract lipids from the membrane. Information

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LANGUAGE: eng

NlmUniqueID: 370626

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Grant and Affiliation Information for SP-A permeabilizes lipopolysaccharide membranes by forming protein aggregates that extract lipids from the membrane.

AFFILIATION: Department of Biochemistry and Molecular Biology I, Complutense University of Madrid, Madrid, Spain.

Country: United States

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MEDLINETA: Biophys J

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