SOS regulation of the type III secretion system of enteropathogenic Escherichia coli.

SOS regulation of the type III secretion system of enteropathogenic Escherichia coli. Research Abstract Details 

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SOS regulation of the type III secretion system of enteropathogenic Escherichia coli. Abstract Text:

Jay L Mellies,Kenneth R Haack,Derek C Galligan,

Genomes of bacterial pathogens contain and coordinately regulate virulence-associated genes in order to cause disease. Enteropathogenic Escherichia coli (EPEC), a major cause of watery diarrhea in infants and a model gram-negative pathogen, expresses a type III secretion system (TTSS) that is encoded by the locus of enterocyte effacement (LEE) and is necessary for causing attaching and effacing intestinal lesions. Effector proteins encoded by the LEE and in cryptic prophage are injected into the host cell cytoplasm by the TTTS apparatus, ultimately leading to diarrhea. The LEE is comprised of multiple polycistronic operons, most of which are controlled by the global, positive regulator Ler. Here we demonstrated that the LEE2 and LEE3 operons also responded to SOS signaling and that this regulation was LexA dependent. As determined by a DNase I protection assay, purified LexA protein bound in vitro to a predicted SOS box located in the divergent, overlapping LEE2/LEE3 promoters. Expression of the lexA1 allele, encoding an uncleavable LexA protein in EPEC, resulted in reduced secretion, particularly in the absence of the Ler regulator. Finally, we obtained evidence that the cryptic phage-located nleA gene encoding an effector molecule is SOS regulated. Thus, we demonstrated, for the first time to our knowledge, that genes encoding components of a TTSS are regulated by the SOS response, and our data might explain how a subset of EPEC effector proteins, encoded in cryptic prophages, are coordinately regulated with the LEE-encoded TTSS necessary for their translocation into host cells.

SOS regulation of the type III secretion system of enteropathogenic Escherichia coli. Publishing Authors By Initials

JL Mellies,KR Haack,DC Galligan,

For similar enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases research abstracts see: enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases research

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SOS regulation of the type III secretion system of enteropathogenic Escherichia coli. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of bacteriology

VOLUME: 189

Page Numbers: 2863-72

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 19

MONTH: 01

YEAR: 2007

SOS regulation of the type III secretion system of enteropathogenic Escherichia coli. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

SOS regulation of the type III secretion system of enteropathogenic Escherichia coli. Keywords Mesh Terms:

KEYWORDS: Serine Endopeptidases

MESH TERMS: metabolism

Chemical & Substance for Abstract: SOS regulation of the type III secretion system of enteropathogenic Escherichia coli. Information

Substance Name: Serine Endopeptidases

Registry Number: EC 3.4.21.-

Grant and Affiliation Information for SOS regulation of the type III secretion system of enteropathogenic Escherichia coli.

AFFILIATION: Biology Department, Reed College, 3203 S.E. Woodstock Blvd., Portland, OR 97202, USA. jay.mellies@reed.edu

Country: United States

AGENCY: United States NIAID

GRANT: R15 AI047802-02

ACRONYM: AI

MEDLINETA: J Bacteriol

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