Sortagging: a versatile method for protein labeling.

Sortagging: a versatile method for protein labeling. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Sortagging: a versatile method for protein labeling. Abstract Text:

Maximilian W Popp,John M Antos,Gijsbert M Grotenbreg,Eric Spooner,Hidde L Ploegh,Maximilian W Popp,John M Antos,Gijsbert M Grotenbreg,Eric Spooner,Hidde L Ploegh,

Genetically encoded reporter constructs that yield fluorescently labeled fusion proteins are a powerful tool for observing cell biological phenomena, but they have limitations. Sortagging (sortase-mediated transpeptidation) is a versatile chemoenzymatic system for site-specific labeling of proteins with small (<2 kDa) probes. Sortagging combines the precision of a genetically encoded tag with the specificity of an enzymatic reaction and the ease and chemical versatility of peptide synthesis. Here we apply this technique to proteins in vitro and on the surface of living cells.

Sortagging: a versatile method for protein labeling. Publishing Authors By Initials

MW Popp,JM Antos,GM Grotenbreg,E Spooner,HL Ploegh,MW Popp,JM Antos,GM Grotenbreg,E Spooner,HL Ploegh,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Sortagging: a versatile method for protein labeling. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Nature chemical biology

VOLUME: 3

Page Numbers: 707-8

Journal Abbreviation: Nat. Chem. Biol.

ISSN: 1552-4469

DAY: 23

MONTH: 09

YEAR: 2007

Sortagging: a versatile method for protein labeling. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101231976

Sortagging: a versatile method for protein labeling. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Sortagging: a versatile method for protein labeling. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Sortagging: a versatile method for protein labeling.

AFFILIATION: Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Nat Chem Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Sortagging: a versatile method for protein labeling Related Publications

• Bridging B cell and T cell recognition of antigen.
• Chemical probes for the rapid detection of Fatty-acylated proteins in Mammalian cells.
• Discovery of CD8+ T cell epitopes in Chlamydia trachomatis infection through use of caged class I MHC tetramers.
• Hidde Ploegh: immunologist, journeyman. Interview by Nicole LeBrasseur.
• Mechanism-based probe for the analysis of cathepsin cysteine proteases in living cells.
• Mechanisms, biology and inhibitors of deubiquitinating enzymes.
• SEL1L nucleates a protein complex required for dislocation of misfolded glycoproteins.
• Sequence and structure evolved separately in a ribosomal ubiquitin variant.
• Sortagging: a versatile method for protein labeling.
• Structure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes.
• The known unknowns of antigen processing and presentation.
• Tubulation of class II MHC compartments is microtubule dependent and involves multiple endolysosomal membrane proteins in primary dendritic cells.
• UNC93B1 delivers nucleotide-sensing toll-like receptors to endolysosomes.