Solution structure of cysteine-rich domain of protein kinase C alpha.

Solution structure of cysteine-rich domain of protein kinase C alpha. Research Abstract Details 

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Solution structure of cysteine-rich domain of protein kinase C alpha. Abstract Text:

S Ichikawa,H Hatanaka,Y Takeuchi,S Ohno,F Inagaki,

The three-dimensional structure of the second cysteine-rich domain of protein kinase C alpha (residues 95-159) was determined in aqueous solution by two-dimensional proton nuclear magnetic resonance and simulated annealing based calculations. On the basis of 687 distance constraints derived from assigned nuclear Overhauser effect (NOE) connectivities, a total of 10 converged structures were obtained from 40 runs of calculations. The atomic root-mean-square (RMS) difference about the mean coordinate positions (excluding residues 1-7, 16-17, 30-34, and 55-65) is 0.55 A for backbone atoms (N, C alpha, C') and 1.07 A for all non-hydrogen atoms. The molecular scaffold is maintained by triple-stranded and double-stranded twisted beta-sheets packed against an alpha-helix and two independent zincs are coordinated by His8, Cys38, Cys41, Cys57 and Cys21, Cys24, His46, Cys49, respectively. It should be noted that the metal ligands from the two sites are interleaved and this is thought to be a new structural motif of a zinc finger domain. Based on the resultant structure, we propose an interaction site of the cysteine-rich domain of protein kinase C with diacylglycerols and phorbol esters.

Solution structure of cysteine-rich domain of protein kinase C alpha. Publishing Authors By Initials

S Ichikawa,H Hatanaka,Y Takeuchi,S Ohno,F Inagaki,

For similar inorganic chemicals: elements: metals, heavy: zinc research abstracts see: inorganic chemicals: elements: metals, heavy: zinc research

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Solution structure of cysteine-rich domain of protein kinase C alpha. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 117

Page Numbers: 566-74

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1995

Solution structure of cysteine-rich domain of protein kinase C alpha. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Solution structure of cysteine-rich domain of protein kinase C alpha. Keywords Mesh Terms:

KEYWORDS: Zinc

MESH TERMS: metabolism

Chemical & Substance for Abstract: Solution structure of cysteine-rich domain of protein kinase C alpha. Information

Substance Name: Protein Kinase C

Registry Number: EC 2.7.11.13

Grant and Affiliation Information for Solution structure of cysteine-rich domain of protein kinase C alpha.

AFFILIATION: Department of Molecular Physiology, The Tokyo Metropolitan Institute of Medical Science.

Country: JAPAN

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MEDLINETA: J Biochem

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