Solution structure of CINC/Gro investigated by heteronuclear NMR.

Solution structure of CINC/Gro investigated by heteronuclear NMR. Research Abstract Details 

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Solution structure of CINC/Gro investigated by heteronuclear NMR. Abstract Text:

H Hanzawa,H Haruyama,K Konishi,K Watanabe,S Tsurufuji,

Cytokine-induced neutrophil chemoattractant (CINC/Gro) is a chemotactic factor for neutrophils in the rat and a member of the CXC chemokine family. The refined three-dimensional structure of CINC/Gro was derived with the aid of heteronuclear magnetic resonance spectroscopy and a hybrid method of distance geometry and simulated annealing. The backbone atomic r.m.s. differences for 19 structures about the mean coordinates for residues 7 to 70 are 0.69+/-0.15 A for the dimer and 0.56+/-0.13 A for the monomer. The N terminal region containing an ELR sequence, an essential motif for chemotactic activity, is disordered in solution, as in other CXC chemokines. The dimer structure consists of a six-stranded anti-parallel beta sheet with two C-terminal alpha helices on the beta sheet. The overall dimer structure of CINC/Gro is similar to that of human MGSA, though the backbone r.m.s.d.s between CINC/Gro and the two MGSA structures were high (1.81 and 2.34 A for the monomer) in spite of the high sequence homology (67%). The major difference resides in the relative position of the C-terminal alpha helix with respect to the beta sheet. This results in a difference of interhelical distances in the dimer: wider (15 A) in CINC/Gro, as in IL-8, than in MGSA (11.7 and 10 A).

Solution structure of CINC/Gro investigated by heteronuclear NMR. Publishing Authors By Initials

H Hanzawa,H Haruyama,K Konishi,K Watanabe,S Tsurufuji,

For similar proteins: recombinant proteins research abstracts see: proteins: recombinant proteins research

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Solution structure of CINC/Gro investigated by heteronuclear NMR. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 123

Page Numbers: 62-70

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1998

Solution structure of CINC/Gro investigated by heteronuclear NMR. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Solution structure of CINC/Gro investigated by heteronuclear NMR. Keywords Mesh Terms:

KEYWORDS: Recombinant Proteins

MESH TERMS: chemistry

Chemical & Substance for Abstract: Solution structure of CINC/Gro investigated by heteronuclear NMR. Information

Substance Name: Recombinant Proteins

Registry Number: 0

Grant and Affiliation Information for Solution structure of CINC/Gro investigated by heteronuclear NMR.

AFFILIATION: Analytical and Metabolic Research Laboratories, Sankyo Co., Ltd., Tokyo.

Country: JAPAN

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MEDLINETA: J Biochem

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