Solution 1h NMR characterization of equilibrium heme orientational disorder with functional consequences in mouse neuroglobin.

Solution 1h NMR characterization of equilibrium heme orientational disorder with functional consequences in mouse neuroglobin. Research Abstract Details 

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Solution 1h NMR characterization of equilibrium heme orientational disorder with functional consequences in mouse neuroglobin. Abstract Text:

Weihong Du,Ray Syvitski,Sylvia Dewilde,Luc Moens,Gerd N La Mar,

The solution 1H NMR spectrum of oxidized (met) mouse neuroglobin, metNgb, demonstrates that it is low-spin and hexacoordinate with strong spectral similarities to ferricytochrome b5. The axial ligands are identified as His(F8) and His(E7), with the latter exhibiting an unstrained Fe-His bond. The presence of two sets of resonances is shown to arise from equilibrium heme orientational isomers ( approximately 2:1). The ligation of cyanide is shown to be extraordinarily slow with a factor approximately 2 difference in rate for the two heme orientations. Not only is Ngb the first mamalian globin with equilibrium heme disorder, but the disorder also has additional functional consequences.

Solution 1h NMR characterization of equilibrium heme orientational disorder with functional consequences in mouse neuroglobin. Publishing Authors By Initials

W Du,R Syvitski,S Dewilde,L Moens,GN La Mar,

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Solution 1h NMR characterization of equilibrium heme orientational disorder with functional consequences in mouse neuroglobin. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 125

Page Numbers: 8080-1

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 9

MONTH: Jul

YEAR: 2003

Solution 1h NMR characterization of equilibrium heme orientational disorder with functional consequences in mouse neuroglobin. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Solution 1h NMR characterization of equilibrium heme orientational disorder with functional consequences in mouse neuroglobin. Keywords Mesh Terms:

KEYWORDS: Solutions

MESH TERMS: chemistry

Chemical & Substance for Abstract: Solution 1h NMR characterization of equilibrium heme orientational disorder with functional consequences in mouse neuroglobin. Information

Substance Name: Globins

Registry Number: 9004-22-2

Grant and Affiliation Information for Solution 1h NMR characterization of equilibrium heme orientational disorder with functional consequences in mouse neuroglobin.

AFFILIATION: Department of Chemistry, University of California-Davis, Davis, CA 95616, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL 16087

ACRONYM: HL

MEDLINETA: J Am Chem Soc

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• Solution 1h NMR characterization of equilibrium heme orientational disorder with functional consequences in mouse neuroglobin.
• [Not Available.]