Soluble CD276 (B7-H3) is released from monocytes, dendritic cells and activated T cells and is detectable in normal human serum.

Soluble CD276 (B7-H3) is released from monocytes, dendritic cells and activated T cells and is detectable in normal human serum. Research Abstract Details 

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Soluble CD276 (B7-H3) is released from monocytes, dendritic cells and activated T cells and is detectable in normal human serum. Abstract Text:

Guangbo Zhang,Jianquan Hou,Jinfang Shi,Gehua Yu,Binfeng Lu,Xueguang Zhang,Guangbo Zhang,Jianquan Hou,Jinfang Shi,Gehua Yu,Binfeng Lu,Xueguang Zhang,

Expression of membrane CD276 (mB7-H3) has been reported on dendritic cells (DCs), monocytes, activated T cells, and various carcinoma cells. However, reports concerning its in vivo function have been inconsistent. Moreover, whether there is a soluble form of this protein is not known. In this study, using a sensitive dual monoclonal antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect the soluble form of B7-H3 (sB7-H3), we demonstrated the release of sB7-H3 by monocytes, DCs, activated T cells, and various mB7-H3+ but not mB7-H3- carcinoma cells. Release from cells was blocked by addition of a matrix metalloproteinase inhibitor (MMPI), which concomitantly caused the accumulation of B7-H3 on the cell surface. To determine the level of circulating sB7-H3, more than 200 serum samples were included in the study. The results indicated that sB7-H3 was present at high levels in all serum samples. Western blotting of sB7-H3 from cell culture supernatants or sera of healthy donors indicated that the molecular size was approximately 16 kDa. Soluble B7-H3 was able to bind to the B7-H3 receptor (B7-H3R) on activated T cells, which showed that sB7-H3 is a functionally active form. These results indicate that release of sB7-H3 from the cell surface is mediated by a matrix metalloproteinase and probably regulates B7-H3R/B7-H3 interactions in vivo. Cleavage of sB7-H3 to an active soluble form would alter both proximal and distal cellular responses.

Soluble CD276 (B7-H3) is released from monocytes, dendritic cells and activated T cells and is detectable in normal human serum. Publishing Authors By Initials

G Zhang,J Hou,J Shi,G Yu,B Lu,X Zhang,G Zhang,J Hou,J Shi,G Yu,B Lu,X Zhang,

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Soluble CD276 (B7-H3) is released from monocytes, dendritic cells and activated T cells and is detectable in normal human serum. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Immunology

VOLUME: 123

Page Numbers: 538-46

Journal Abbreviation: Immunology

ISSN: 1365-2567

DAY: 9

MONTH: 01

YEAR: 2008

Soluble CD276 (B7-H3) is released from monocytes, dendritic cells and activated T cells and is detectable in normal human serum. Information

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LANGUAGE: eng

NlmUniqueID: 374672

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Grant and Affiliation Information for Soluble CD276 (B7-H3) is released from monocytes, dendritic cells and activated T cells and is detectable in normal human serum.

AFFILIATION: Clinical Immunology Laboratory, Suzhou University no. 1 Affiliated Hospital, Suzhou, China.

Country: England

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MEDLINETA: Immunology

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