Solubilized human zona pellucida competes with a fucosylated neoglycoprotein for binding sites on the human sperm surface.

Solubilized human zona pellucida competes with a fucosylated neoglycoprotein for binding sites on the human sperm surface. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Solubilized human zona pellucida competes with a fucosylated neoglycoprotein for binding sites on the human sperm surface. Abstract Text:

OBJECTIVE: To evaluate the implication of fucose binding sites on the sperm surface in the sperm-zona pellucida (ZP) binding by examining the ability of a fucosylated neoglycoprotein to compete with solubilized zona proteins for sperm binding. DESIGN: The binding of a fucosylated neoglycoprotein to spermatozoa was analyzed in relation to sperm capacitation and the actual acrosomal status. The effect of solubilized ZP material on the binding was examined. SETTING: Private hospital, public research center, and university department. PATIENTS, PARTICIPANTS: Healthy volunteers with normal sperm parameters. INTERVENTIONS: None. MAIN OUTCOME MEASURES: Fluorescence signal detected by fluorescence-activated cell sorting or fluorescence microscopy on spermatozoa incubated with fluorescein isothiocyanate-labeled fucosylated neoglycoprotein. RESULTS: There was a small subpopulation of spermatozoa that showed a strong binding activity for the fucosylated neoglycoprotein, and the size of this subpopulation increased with the time of exposure to capacitating conditions. The binding sites were located on the head (mainly on the acrosomal region) of acrosome-intact spermatozoa. The neoglycoprotein binding was competitively inhibited by solubilized ZP. CONCLUSIONS: The data show that a commercially available fucosylated neoglycoprotein competes with solubilized ZP proteins for binding sites on the sperm surface. Further research into the relationship between the neoglycoprotein binding and zona binding is warranted to evaluate the potential diagnostic usefulness of this neoglycoprotein.

Solubilized human zona pellucida competes with a fucosylated neoglycoprotein for binding sites on the human sperm surface. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Solubilized human zona pellucida competes with a fucosylated neoglycoprotein for binding sites on the human sperm surface. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Fertility and sterility

VOLUME: 60

Page Numbers: 344-50

Journal Abbreviation: Fertil. Steril.

ISSN: 0015-0282

DAY: 19

MONTH: Aug

YEAR: 1993

Solubilized human zona pellucida competes with a fucosylated neoglycoprotein for binding sites on the human sperm surface. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372772

Solubilized human zona pellucida competes with a fucosylated neoglycoprotein for binding sites on the human sperm surface. Keywords Mesh Terms:

KEYWORDS: Zona Pellucida

MESH TERMS: metabolism

Chemical & Substance for Abstract: Solubilized human zona pellucida competes with a fucosylated neoglycoprotein for binding sites on the human sperm surface. Information

Substance Name: Fucose

Registry Number: 3713-31-3

Grant and Affiliation Information for Solubilized human zona pellucida competes with a fucosylated neoglycoprotein for binding sites on the human sperm surface.

AFFILIATION: Center for Reproductive Biology and Medicine, American Hospital of Paris, France.

Country: UNITED STATES

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Fertil Steril

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Solubilized human zona pellucida competes with a fucosylated neoglycoprotein for binding sites on the human sperm surface Related Publications

• Active ingredients of substance use-focused self-help groups.
• Central anticholinergic syndrome: a case report.
• Conversation with Rudolf moos.
• Determinants of functioning and well-being among individuals with schizophrenia: an integrated model.
• Direct detection of formaldehyde in air by a novel NAD+- and glutathione-independent formaldehyde dehydrogenase-based biosensor.
• Effect of slow cathode rays on microorganisms.
• Emergent synchronous bursting of oxytocin neuronal network.
• Functioning status of adult children of depressed parents: a 23-year follow-up.
• Imaging performance of telescope mirrors for far-ultraviolet astronomy.
• Opposite potentiality of hypothalamic coexpressed neuropeptides, apelin and vasopressin in maintaining body-fluid homeostasis.
• Oxidative stress and damage in liver, but not in brain, of Fischer 344 rats subjected to dietary iron supplementation with lipid-soluble [(3,5,5-trimethylhexanoyl)ferrocene].
• Participation in specific treatment components predicts alcohol-specific and general coping skills.
• Personal and treatment-related predictors of abstinence self-efficacy.
• Predictors of 4-year outcome of community residential treatment for patients with substance use disorders.
• Predictors of changes in alcohol-related self-efficacy over 16 years.
• Predictors of outcome for patients with substance-use disorders five years after treatment dropout.
• Problem-free drinking over 16 years among individuals with alcohol use disorders.
• Protective resources and long-term recovery from alcohol use disorders.
• Screening psychiatric patients for illicit drug use disorders and problems.
• Solubilized human zona pellucida competes with a fucosylated neoglycoprotein for binding sites on the human sperm surface.
• Spatial imaging in the soft x-ray region (20-304 A) utilizing the astigmatism of a grazing incidence concave grating.
• Spirituality/religiosity promotes acceptance-based responding and 12-step involvement.
• Substance misuse among older adults: a neglected but treatable problem.
• Substance use disorder treatment programs in Switzerland and the USA: Program characteristics and 1-year outcomes.
• The basic aspects of radiation therapy.
• Treated and untreated alcohol-use disorders: course and predictors of remission and relapse.
• Upward pressures on drinking: exposure and reactivity in adulthood.
• Vg1 has specific processing requirements that restrict its action to body axis patterning centers.
• [Commentary] CONTEXT AND MECHANISMS OF REACTIVITY TO ASSESSMENT AND TREATMENT.
• [Not Available.]