Solid-state 2H NMR structure of retinal in metarhodopsin I.

Solid-state 2H NMR structure of retinal in metarhodopsin I. Research Abstract Details 

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Solid-state 2H NMR structure of retinal in metarhodopsin I. Abstract Text:

Gilmar F J Salgado,Andrey V Struts,Katsunori Tanaka,Sonja Krane,Koji Nakanishi,Michael F Brown,

The structural and photochemical changes in rhodopsin due to absorption of light are crucial for understanding the process of visual signaling. We investigated the structure of trans-retinal in the metarhodopsin I photointermediate (MI), where the retinylidene cofactor functions as an antagonist. Rhodopsin was regenerated using retinal that was (2)H-labeled at the C5, C9, or C13 methyl groups and was reconstituted with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine. Membranes were aligned by isopotential centrifugation, and rhodopsin in the supported bilayers was then bleached and cryotrapped in the MI state. Solid-state (2)H NMR spectra of oriented rhodopsin in the low-temperature lipid gel state were analyzed in terms of a static uniaxial distribution (Nevzorov, A. A.; Moltke, S.; Heyn, M. P.; Brown, M. F. J. Am. Chem. Soc. 1999, 121, 7636-7643). The line shape analysis allowed us to obtain the methyl bond orientations relative to the membrane normal in the presence of substantial alignment disorder (mosaic spread). Relative orientations of the methyl groups were used to calculate effective torsional angles between the three different planes that represent the polyene chain and the beta-ionone ring of retinal. Assuming a three-plane model, a less distorted structure was found for retinal in MI compared to the dark state. Our results are pertinent to how photonic energy is channeled within the protein to allow the strained retinal conformation to relax, thereby forming the activated state of the receptor.

Solid-state 2H NMR structure of retinal in metarhodopsin I. Publishing Authors By Initials

GF Salgado,AV Struts,K Tanaka,S Krane,K Nakanishi,MF Brown,

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Solid-state 2H NMR structure of retinal in metarhodopsin I. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 128

Page Numbers: 11067-71

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ISSN: 0002-7863

DAY: 30

MONTH: Aug

YEAR: 2006

Solid-state 2H NMR structure of retinal in metarhodopsin I. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Solid-state 2H NMR structure of retinal in metarhodopsin I.

AFFILIATION: Department of Biochemistry & Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA.

Country: United States

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MEDLINETA: J Am Chem Soc

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