Sodium/calcium exchanger expression in the mouse and rat olfactory systems.

Sodium/calcium exchanger expression in the mouse and rat olfactory systems. Research Abstract Details 

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Sodium/calcium exchanger expression in the mouse and rat olfactory systems. Abstract Text:

Martina Pyrski,Jae Hyung Koo,Swamy K Polumuri,Abdul M Ruknudin,Joyce W Margolis,Dan H Schulze,Frank L Margolis,

Sodium/calcium (Na(+)/Ca(2+)) exchangers are membrane transport systems that regulate Ca(2+)-homeostasis in many eukaryotic cells. In olfactory and vomeronasal sensory neurons ligand-induced olfactory signal transduction is associated with influx and elevation of intracellular Ca(2+), [Ca(2+)](i). While much effort has been devoted to the characterization of Ca(2+)-related excitation and adaptation events of olfactory chemosensory neurons (OSNs), much less is known about mechanisms that return [Ca(2+)](i) to the resting state. To identify proteins participating in the poststimulus Ca(2+)-clearance of mouse OSNs, we analyzed the expression of three potassium (K(+))-independent (NCX1, 2, 3) and three K(+)-dependent (NCKX1, 2, 3) Na(+)/Ca(2+) exchangers. In situ hybridization showed that mRNAs of all six Na(+)/Ca(2+) exchangers coexist in neurons of the olfactory and vomeronasal systems, and that some are already detectable in the embryo. Of these, NCX1 and NCKX1 represent the most and least abundant mRNAs, respectively. Moreover, immunohistochemistry revealed that the NCX1, 2, and 3 proteins are expressed in nearly all neurons of the olfactory epithelium, the vomeronasal organ, the septal organ of Masera, and the Grueneberg ganglion. These three exchanger proteins display different expression profiles in dendrites, knobs, and plasma membranes of OSNs and in sustentacular cells. Furthermore, we show that NCX1 mRNA in rat olfactory mucosa is expressed as 8 alternative splice variants. This is the first comprehensive analysis of Na(+)/Ca(2+) exchanger expression in the mammalian olfactory system. Our results suggest that Ca(2+)-extrusion by OSNs utilizes multiple different Na(+)/Ca(2+) exchangers and that different subtypes are targeted to different subcellular compartments.

Sodium/calcium exchanger expression in the mouse and rat olfactory systems. Publishing Authors By Initials

M Pyrski,JH Koo,SK Polumuri,AM Ruknudin,JW Margolis,DH Schulze,FL Margolis,

For similar vomeronasal organ research abstracts see: vomeronasal organ research

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Sodium/calcium exchanger expression in the mouse and rat olfactory systems. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of comparative neurology

VOLUME: 501

Page Numbers: 944-58

Journal Abbreviation: J. Comp. Neurol.

ISSN: 0021-9967

DAY: 20

MONTH: Apr

YEAR: 2007

Sodium/calcium exchanger expression in the mouse and rat olfactory systems. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 406041

Sodium/calcium exchanger expression in the mouse and rat olfactory systems. Keywords Mesh Terms:

KEYWORDS: Vomeronasal Organ

MESH TERMS: metabolism

Chemical & Substance for Abstract: Sodium/calcium exchanger expression in the mouse and rat olfactory systems. Information

Substance Name: Calcium

Registry Number: 7440-70-2

Grant and Affiliation Information for Sodium/calcium exchanger expression in the mouse and rat olfactory systems.

AFFILIATION: Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL-62521

ACRONYM: HL

MEDLINETA: J Comp Neurol

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