SNARE status regulates tether recruitment and function in homotypic COPII vesicle fusion.

SNARE status regulates tether recruitment and function in homotypic COPII vesicle fusion. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

SNARE status regulates tether recruitment and function in homotypic COPII vesicle fusion. Abstract Text:

Marvin Bentley,Yingjian Liang,Karl Mullen,Dalu Xu,Elizabeth Sztul,Jesse C Hay,

In mammals, coat complex II (COPII)-coated transport vesicles deliver secretory cargo to vesicular tubular clusters (VTCs) that facilitate cargo sorting and transport to the Golgi. We documented in vitro tethering and SNARE-dependent homotypic fusion of endoplasmic reticulum-derived COPII transport vesicles to form larger cargo containers characteristic of VTCs ( Xu, D., and Hay, J. C. (2004) J. Cell Biol. 167, 997-1003). COPII vesicles thus appear to contain all necessary components for homotypic tethering and fusion, providing a pathway for de novo VTC biogenesis. Here we demonstrate that antibodies against the endoplasmic reticulum/Golgi SNARE Syntaxin 5 inhibit COPII vesicle homotypic tethering as well as fusion, implying an unanticipated role for SNAREs upstream of fusion. Inhibition of SNARE complex access and/or disassembly with dominant-negative alpha-soluble NSF attachment protein (SNAP) also inhibited tethering, implicating SNARE status as a critical determinant in COPII vesicle tethering. The tethering-defective vesicles generated in the presence of dominant-negative alpha-SNAP specifically lacked the Rab1 effectors p115 and GM130 but not other peripheral membrane proteins. Furthermore, Rab effectors, including p115, were shown to be required for homotypic COPII vesicle tethering. Thus, our results demonstrate a requirement for SNARE-dependent tether recruitment and function in COPII vesicle fusion. We anticipate that recruitment of tether molecules by an upstream SNARE signal ensures that tethering events are initiated only at focal sites containing appropriately poised fusion machinery.

SNARE status regulates tether recruitment and function in homotypic COPII vesicle fusion. Publishing Authors By Initials

M Bentley,Y Liang,K Mullen,D Xu,E Sztul,JC Hay,

For similar proteins: membrane proteins: membrane fusion proteins: snare proteins research abstracts see: proteins: membrane proteins: membrane fusion proteins: snare proteins research

PUBMED ID PMID:

MEDLINE DATE:

SNARE status regulates tether recruitment and function in homotypic COPII vesicle fusion. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 281

Page Numbers: 38825-33

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 12

MONTH: 10

YEAR: 2006

SNARE status regulates tether recruitment and function in homotypic COPII vesicle fusion. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

SNARE status regulates tether recruitment and function in homotypic COPII vesicle fusion. Keywords Mesh Terms:

KEYWORDS: SNARE Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: SNARE status regulates tether recruitment and function in homotypic COPII vesicle fusion. Information

Substance Name: SNARE Proteins

Registry Number: 0

Grant and Affiliation Information for SNARE status regulates tether recruitment and function in homotypic COPII vesicle fusion.

AFFILIATION: Division of Biological Sciences, University of Montana, Missoula, MT 59812-4824, USA.

Country: United States

AGENCY: United States NCRR

GRANT: RR 015583

ACRONYM: RR

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

SNARE status regulates tether recruitment and function in homotypic COPII vesicle fusion Related Publications

• A synthetic maternal-effect selfish genetic element drives population replacement in Drosophila.
• Bacterial degradation of N,N-diethyl-m-toluamide (DEET): cloning and heterologous expression of DEET hydrolase.
• Calcium: a fundamental regulator of intracellular membrane fusion?
• Characteristics and zona binding ability of FRESH and cooled domestic cat epididymal spermatozoa.
• Chemically mediated competition between microbes and animals: microbes as consumers in food webs.
• Chronic fetal hypoglycemia inhibits the later steps of stimulus-secretion coupling in pancreatic beta-cells.
• Climatic similarity and biological exchange in the worldwide airline transportation network.
• Defining the global spatial limits of malaria transmission in 2005.
• Deletion 22q11: spectrum of associated disorders.
• Early postnatal nutritional requirements of the very preterm infant based on a presentation at the NICHD-AAP workshop on research in neonatology.
• Economic modeling and sensitivity analysis.
• Effect of hyperinsulinemia on amino acid utilization and oxidation independent of glucose metabolism in the ovine fetus.
• Estrogen receptor-alpha mediates estrogen protection from angiotensin II-induced hypertension in conscious female mice.
• Evaluating the CSAPPA subscales as potential screening instruments for developmental coordination disorder.
• Fractures and avascular necrosis before and after orthotopic liver transplantation: long-term follow-up and predictive factors.
• Global transport networks and infectious disease spread.
• How do changes in tumor matrix affect the outcome of virotherapy?
• Human matrix metalloproteinase-8 gene delivery increases the oncolytic activity of a replicating adenovirus.
• Hypoglycemia in newborn infants: Features associated with adverse outcomes.
• Potential impacts of nonalgal materials on water-leaving Sun induced chlorophyll fluorescence signals in coastal waters.
• Predator release of the gastropod Cyphoma gibbosum increases predation on gorgonian corals.
• Prophylaxis in adults with haemophilia.
• Risk management: the top 10 mistakes dentists make.
• SNARE status regulates tether recruitment and function in homotypic COPII vesicle fusion.
• Sex differences in angiotensin II- induced hypertension.
• Skin toxicology.
• Stille coupling reactions in the synthesis of hypoxia-selective 3-alkyl-1,2,4-benzotriazine 1,4-dioxide anticancer agents.
• The Drosophila caspase Ice is important for many apoptotic cell deaths and for spermatid individualization, a nonapoptotic process.
• The gradual emergence of sex differences in aggression: alternative hypotheses.
• echinus, required for interommatidial cell sorting and cell death in the Drosophila pupal retina, encodes a protein with homology to ubiquitin-specific proteases.