Small-scale analysis of O-linked oligosaccharides from glycoproteins and mucins separated by gel electrophoresis.

Small-scale analysis of O-linked oligosaccharides from glycoproteins and mucins separated by gel electrophoresis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Small-scale analysis of O-linked oligosaccharides from glycoproteins and mucins separated by gel electrophoresis. Abstract Text:

Benjamin L Schulz,Nicolle H Packer,Niclas G Karlsson,

A technique with subpicomolar sensitivity was developed for analyzing O-linked oligosaccharides released from glycoproteins separated by gel electrophoresis. The protocol involves gel electrophoresis, electroblotting to poly-(vinylidene fluoride) membrane, reductive beta-elimination, and analysis of released oligosaccharides by liquid chromatography coupled to negative ion electrospray mass spectrometry. It was also found that N-linked oligosaccharides could be recovered under the same conditions, found both as free oligosaccharides and as distinct glycopeptides created from reductive cleavage of the protein backbone, giving some information on site-specific glycosylation. The method was used to demonstrate that the difference between human alpha-2HS-glycoprotein isoforms separated by 2D-gel electrophoresis was partially due to sialylation of both O-linked and N-linked oligosaccharides. It was also shown that both acidic and neutral oligosaccharides could be recovered and analyzed simultaneously from high molecular mass (200,000-5,000,000 Da) highly glycosylated mucin glycoproteins collected from small intestine and saliva and separated by sodium dodecyl sulfate-agarose/polyacrylamide composite gels. Mass spectrometric data not only gave information about the mass distribution of the heterogeneous mixtures of oligosaccharides from [M - xH](x-) ions but also gave information about the isomeric heterogeneity of the oligosaccharides from their resolution by porous graphitized carbon chromatography. Tandem mass spectrometry was explored as a technique for distinguishing between oligosaccharide isomers with different sequences and also between oligosaccharides with the same sequence but with different linkage configurations.

Small-scale analysis of O-linked oligosaccharides from glycoproteins and mucins separated by gel electrophoresis. Publishing Authors By Initials

BL Schulz,NH Packer,NG Karlsson,

For similar proteins: fetal proteins: alpha-fetoproteins research abstracts see: proteins: fetal proteins: alpha-fetoproteins research

PUBMED ID PMID:

MEDLINE DATE:

Small-scale analysis of O-linked oligosaccharides from glycoproteins and mucins separated by gel electrophoresis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical chemistry

VOLUME: 74

Page Numbers: 6088-97

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 1

MONTH: Dec

YEAR: 2002

Small-scale analysis of O-linked oligosaccharides from glycoproteins and mucins separated by gel electrophoresis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370536

Small-scale analysis of O-linked oligosaccharides from glycoproteins and mucins separated by gel electrophoresis. Keywords Mesh Terms:

KEYWORDS: alpha-Fetoproteins

MESH TERMS: analysis

Chemical & Substance for Abstract: Small-scale analysis of O-linked oligosaccharides from glycoproteins and mucins separated by gel electrophoresis. Information

Substance Name: alpha2HS glycoprotein

Registry Number: 0

Grant and Affiliation Information for Small-scale analysis of O-linked oligosaccharides from glycoproteins and mucins separated by gel electrophoresis.

AFFILIATION: Proteome Systems Ltd., Locked Bag 2073, North Ryde Sydney, NSW 1670 Australia. benjamin.schulz@proteomesystems.com

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Anal Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Small-scale analysis of O-linked oligosaccharides from glycoproteins and mucins separated by gel electrophoresis Related Publications

• A flexible measurement system for physiological signals in mobile health care.
• A major difference between transdetermination and homeosis.
• A monocyte-specific peptide from herpes simplex virus type 2 glycoprotein G activates the NADPH-oxidase but not chemotaxis through a G-protein-coupled receptor distinct from the members of the formyl peptide receptor family.
• A new approach for studying correlations between the chemical structure and the rheological properties in carboxymethyl cellulose.
• Adaptive spatio-temporal filtration of bioelectrical signals.
• An integrated glucose-insulin model to describe oral glucose tolerance test data in type 2 diabetics.
• An integrated model for the effect of budesonide on ACTH and cortisol in healthy volunteers.
• Analytical strategies for the quality assessment of recycled high-impact polystyrene: a combination of thermal analysis, vibrational spectroscopy, and chromatography.
• Characterization of gangliosides of epithelial cells of calf small intestine, with special reference to receptor-active sequences for enteropathogenic Escherichia coli K99.
• Clinical use and material wear of polymeric tracheostomy tubes.
• Conditional weighted residuals (CWRES): a model diagnostic for the FOCE method.
• Drying rate variations of latex dispersions due to salt induced skin formation.
• Efficient nanosecond optical parametric oscillators based on periodically poled KTP emitting in the 1.8-2.5-mum spectral region.
• First-order type II quasi-phase-matched UV generation in periodically poled KTP.
• Glycosylation of sputum mucins is altered in cystic fibrosis patients.
• Hydraulic and mechanical properties of young Norway spruce clones related to growth and wood structure.
• Implementation of a transit compartment model for describing drug absorption in pharmacokinetic studies.
• Mitochondrial expression of the Drosophila melanogaster multisubstrate deoxyribonucleoside kinase.
• Murine models of acute neuronopathic Gaucher disease.
• Nonoperatively treated burst fractures of the thoracic and lumbar spine in adults: a 23- to 41-year follow-up.
• Personality disorders and change in personality.
• Polarization mode dispersion induced pulse broadening in optical fibers.
• Reduced-order modeling, error estimation, and the role of the start-vector: the recursive residue generation method revisited.
• Short-term effects of metformin in type 2 diabetes.
• Small-scale analysis of O-linked oligosaccharides from glycoproteins and mucins separated by gel electrophoresis.
• Spectral characterization of human EHG frequency components based on the extraction and reconstruction of the ridges in the scalogram.
• Statistical aspects of van't Hoff analysis: a simulation study.
• Temporal relationship between infestation with lice (Bovicola ovis Schrank) and the development of pruritic behaviour and fleece derangement in sheep.
• Variations in the axis of motion during head repositioning--a comparison of subjects with whiplash-associated disorders or non-specific neck pain and healthy controls.
• Vulnerability to psychosis increases the risk of depression. Results of the RADEP study.