Small molecule regulators of autophagy identified by an image-based high-throughput screen.

Small molecule regulators of autophagy identified by an image-based high-throughput screen. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Small molecule regulators of autophagy identified by an image-based high-throughput screen. Abstract Text:

Lihong Zhang,Jia Yu,Heling Pan,Ping Hu,Yan Hao,Wenqing Cai,Hong Zhu,Albert D Yu,Xin Xie,Dawei Ma,Junying Yuan,Lihong Zhang,Jia Yu,Heling Pan,Ping Hu,Yan Hao,Wenqing Cai,Hong Zhu,Albert D Yu,Xin Xie,Dawei Ma,Junying Yuan,

Autophagy is a lysosome-dependent cellular catabolic mechanism mediating the turnover of intracellular organelles and long-lived proteins. Reduction of autophagy activity has been shown to lead to the accumulation of misfolded proteins in neurons and may be involved in chronic neurodegenerative diseases such as Huntington's disease and Alzheimer's disease. To explore the mechanism of autophagy and identify small molecules that can activate it, we developed a series of high-throughput image-based screens for small-molecule regulators of autophagy. This series of screens allowed us to distinguish compounds that can truly induce autophagic degradation from those that induce the accumulation of autophagosomes as a result of causing cellular damage or blocking downstream lysosomal functions. Our analyses led to the identification of eight compounds that can induce autophagy and promote long-lived protein degradation. Interestingly, seven of eight compounds are FDA-approved drugs for treatment of human diseases. Furthermore, we show that these compounds can reduce the levels of expanded polyglutamine repeats in cultured cells. Our studies suggest the possibility that some of these drugs may be useful for the treatment of Huntington's and other human diseases associated with the accumulation of misfolded proteins.

Small molecule regulators of autophagy identified by an image-based high-throughput screen. Publishing Authors By Initials

L Zhang,J Yu,H Pan,P Hu,Y Hao,W Cai,H Zhu,AD Yu,X Xie,D Ma,J Yuan,L Zhang,J Yu,H Pan,P Hu,Y Hao,W Cai,H Zhu,AD Yu,X Xie,D Ma,J Yuan,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Small molecule regulators of autophagy identified by an image-based high-throughput screen. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 19023-8

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 1091-6490

DAY: 16

MONTH: 11

YEAR: 2007

Small molecule regulators of autophagy identified by an image-based high-throughput screen. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Small molecule regulators of autophagy identified by an image-based high-throughput screen. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Small molecule regulators of autophagy identified by an image-based high-throughput screen. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Small molecule regulators of autophagy identified by an image-based high-throughput screen.

AFFILIATION: State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 354 Fenglin Road, Shanghai 200032, China.

Country: United States

AGENCY: United States NIA

GRANT: R37 AG12859

ACRONYM: AG

MEDLINETA: Proc Natl Acad Sci U S A

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Small molecule regulators of autophagy identified by an image-based high-throughput screen Related Publications

• A remote controlled multimode micro-stimulator for freely moving animals.
• Bone morphogenetic proteins induce apoptosis in human pulmonary vascular smooth muscle cells.
• Experimental study on effects of vitamin B(6) on dexamethason-induced palatal cleft formation in the rat.
• Pentacyclic Triterpenoids from Leaves of Excoecaria agallocha.
• Steroid withdrawal in the mouse results in anxiogenic effects of 3alpha,5beta-THP: a possible model of premenstrual dysphoric disorder.
• [Changes in the phosphorylation of cAMP response element binding protein in the rat nucleus accumbens after acute and chronic ethanol administration]
• [Clinical features in 4 Chinese families with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).]
• [Clinical pathology and molecular genetics on familial amyloidotic polyneuropathy]
• [Clinical study on improving the diagnostic criteria for neonatal asphyxia]
• [Comparing loss of anterior column height and cervical Cobb angle with three different types of anterior cervical discectomy and fusion]
• [Detection of desmosine in elastin by high performance liquid chromatography (HPLC)]
• [Development of a portable glucose meter based on PIC microcomputer]
• [Effects of platelet-rich plasma on proliferation and osteogenetic activity of marrow mesenchymal stem cells in vitro]
• [Effects of urotensin II on isolated rat hearts under normal perfusion and ischemia reperfusion]
• [Expression of midkine in eutopic and ectopic endometrium of patients with severe endometriosis and its clinical significance]
• [Fermentation process monitoring and fault detection based on dynamic MPCA]
• [Isolation of endophytic bacteria in potato and test of antagonistic action to bacterial ring rot of potato]
• [Measuring trace germanium in vegetables with the spectrofluorometric method]
• [Pelvic artery representation on three-dimensional reconstructed multislice spiral CT images: variability between the young and the elderly]
• [Photoluminescence and UV-Vis absorption spectra characteristics of metal complexes of 8-hydroxyquinoline]
• [Properties and advance of hepcidin]
• [Research and advancement of image-guided radiotherapy]
• [Significance of serum level of NO and IL-8 in helicobacter pylori associated gastric diseases]
• [Space flight and peroxidative damage]
• [Spectral properties of broad emission bands of Cr3+ in A3M2Ge3O12 (A = Cd2+, Ca2+; M = AI3+, Ga3+, Sc3+) garnets]
• [Spectral study of the conformational change of yeast alcohol dehydrogenase induced by alcohol]
• [Study of the volatilization inhibitor for formalin]
• [Synthesis and spectral properties of tailed sercine tetraphenylporphyrin cobalt complex]
• [The antimicrobial effects of chitosans on oral pathogenic microbes in vitro]
• [The evolvement of the theory of dental implant-bone interface]