Slow-onset feedback inhibition: inhibition of Mycobacterium tuberculosis alpha-isopropylmalate synthase by L-leucine.

Slow-onset feedback inhibition: inhibition of Mycobacterium tuberculosis alpha-isopropylmalate synthase by L-leucine. Research Abstract Details 

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Slow-onset feedback inhibition: inhibition of Mycobacterium tuberculosis alpha-isopropylmalate synthase by L-leucine. Abstract Text:

Luiz P S de Carvalho,Argyrides Argyrou,John S Blanchard,

This report describes the first demonstration of slow-onset feedback inhibition of an enzyme that catalyzes the first committed step in a biosynthetic pathway. alpha-Isopropylmalate synthase (IPMS) catalyzes the first committed step of the l-leucine biosynthetic pathway and is feedback-inhibited by l-leucine. Initial velocity experiments on the Mycobacterium tuberculosis IPMS indicate that inhibition by l-leucine is linearly noncompetitive versus alpha-ketoisovalerate. Time-courses displayed a burst of product formation followed by a linear steady-state rate when reactions were initiated by the addition of enzyme. The burst rate showed a hyperbolic dependence on the concentration of l-leucine indicating that inhibition proceeds in two steps, an initial rapid binding step followed by slow isomerization to a more tightly bound complex.

Slow-onset feedback inhibition: inhibition of Mycobacterium tuberculosis alpha-isopropylmalate synthase by L-leucine. Publishing Authors By Initials

LP de Carvalho,A Argyrou,JS Blanchard,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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Slow-onset feedback inhibition: inhibition of Mycobacterium tuberculosis alpha-isopropylmalate synthase by L-leucine. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 127

Page Numbers: 10004-5

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 20

MONTH: Jul

YEAR: 2005

Slow-onset feedback inhibition: inhibition of Mycobacterium tuberculosis alpha-isopropylmalate synthase by L-leucine. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Slow-onset feedback inhibition: inhibition of Mycobacterium tuberculosis alpha-isopropylmalate synthase by L-leucine. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: enzymology

Chemical & Substance for Abstract: Slow-onset feedback inhibition: inhibition of Mycobacterium tuberculosis alpha-isopropylmalate synthase by L-leucine. Information

Substance Name: 2-Isopropylmalate Synthase

Registry Number: EC 2.3.3.13

Grant and Affiliation Information for Slow-onset feedback inhibition: inhibition of Mycobacterium tuberculosis alpha-isopropylmalate synthase by L-leucine.

AFFILIATION: Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, 10461, USA.

Country: United States

AGENCY: United States PHS

GRANT: NIH A133696

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MEDLINETA: J Am Chem Soc

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