Skeletal muscle fiber type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression in fed and food-deprived rats.

Skeletal muscle fiber type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression in fed and food-deprived rats. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Skeletal muscle fiber type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression in fed and food-deprived rats. Abstract Text:

Paul J Leblanc,Robert A Harris,Sandra J Peters,

Fiber type specificity of pyruvate dehydrogenase (PDH) phosphatase (PDP) was determined in fed (CON) and 48-h food-deprived (FD) rats. PDP activity and isoform protein content were determined in soleus (slow-twitch oxidative), red gastrocnemius (RG; fast-twitch oxidative glycolytic), and white gastrocnemius (WG; fast-twitch glycolytic) muscles. When normalized for mitochondrial volume, there was no difference in PDP activity between muscle types or CON and FD. When expressed per gram wet tissue weight, PDP activity was higher in RG compared with soleus and WG in both CON and FD rats. PDP activities from CON muscles were 1.48 +/- 0.19, 2.68 +/- 0.65, and 1.20 +/- 0.33 nmol x min(-1) x g wet tissue wt(-1) in soleus, RG, and WG, respectively, and decreased in FD muscles (1.22 +/- 0.22, 2.00 +/- 0.57, and 0.84 +/- 0.18 nmol x min(-1) x g wet tissue wt(-1)). This correlated with increased PDP2 protein, however, only in RG, as PDP2 was not detectable in soleus or WG. PDP1 protein was not responsive to food deprivation in all fiber types. In conclusion, PDP activity and protein content were higher in fast-twitch oxidative glycolytic muscles from CON and FD rats, identifying a unique inter- and intramuscular distribution. FD induced a small but significant decrease in PDP activity that was partially due to decreases in PDP2 protein. As a result, coordinate changes to PDP activity opposite to those of the other regulatory enzyme, PDH kinase, during food deprivation would maximize the inactivation of skeletal muscle PDH and enhance carbohydrate conservation during periods of limited carbohydrate supply.

Skeletal muscle fiber type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression in fed and food-deprived rats. Publishing Authors By Initials

PJ Leblanc,RA Harris,SJ Peters,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, sprague-dawley research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, sprague-dawley research

PUBMED ID PMID:

MEDLINE DATE:

Skeletal muscle fiber type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression in fed and food-deprived rats. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Endocrinology and

VOLUME: 292

Page Numbers: E571-6

Journal Abbreviation: Am. J. Physiol. Endocrinol. Me

ISSN: 0193-1849

DAY: 3

MONTH: 10

YEAR: 2006

Skeletal muscle fiber type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression in fed and food-deprived rats. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901226

Skeletal muscle fiber type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression in fed and food-deprived rats. Keywords Mesh Terms:

KEYWORDS: Rats, Sprague-Dawley

MESH TERMS: metabolism

Chemical & Substance for Abstract: Skeletal muscle fiber type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression in fed and food-deprived rats. Information

Substance Name: Pyruvate Dehydrogenase (Lipoamide)-Phosp

Registry Number: EC 3.1.3.43

Grant and Affiliation Information for Skeletal muscle fiber type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression in fed and food-deprived rats.

AFFILIATION: Faculty of Applied Health Sciences, Brock University, 500 Glenridge Ave., St. Catharines, ON, Canada L2S 3A1. pleblanc@brocku.ca

Country: United States

AGENCY: United States NIDDK

GRANT: DK-47844

ACRONYM: DK

MEDLINETA: Am J Physiol Endocrinol Metab

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Skeletal muscle fiber type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression in fed and food-deprived rats Related Publications

• A mouse TRAPP-related protein is involved in pigmentation.
• Assessment of the factors affecting the volume of fetally derived tissue in the bovine placentome.
• Calcitriol protects against the dopamine- and serotonin-depleting effects of neurotoxic doses of methamphetamine.
• Catalytic receptors.
• Cell-surface transmitter transporters.
• Commentary left and right in classical Greece and Italy.
• Cooperative learning: a new application of problem-based learning in mental health training.
• De novo malignancies following liver transplantation: a case-control study with long-term follow-up.
• Determination of etoposide (VP16-213) and teniposide (VM-26) in serum by high-performance liquid chromatography with electrochemical detection.
• Dog ownership enhances symptomatic responses to air pollution in children with asthma.
• Fe(I)-mediated reductive cleavage and coupling of CO(2): an Fe(II)(mu-O, mu-CO)Fe(II) core.
• Functional characterization of the rice kaurene synthase-like gene family.
• Guide to Receptors and Channels, 2nd edition (2007 Revision).
• Immune epitope mapping in the post-genomic era: lessons for vaccine development.
• Long-term consequences of pain in human neonates.
• Nanoscopic medicine: the next frontier.
• Neuromodulation for the treatment of refractory interstitial cystitis.
• Novel model of antigen-specific induction of bile duct injury.
• Nuclear receptors.
• On the feasibility of N2 fixation via a single-site FeI/FeIV cycle: Spectroscopic studies of FeI(N2)FeI, FeIV[triple bond]N, and related species.
• Polymorphism analysis of six selenoprotein genes: support for a selective sweep at the glutathione peroxidase 1 locus (3p21) in Asian populations.
• Prospective study of air pollution and bronchitic symptoms in children with asthma.
• Pseudotetrahedral manganese complexes supported by the anionic tris(phosphino)borate ligand [PhBP(iPr)3].
• Regular smoking and asthma incidence in adolescents.
• Separation of ribonucleotides, deoxynucleotides, cyclic nucleotides and deoxycyclic nucleotides by reversed-phase high-performance liquid chromatography.
• Severe acute respiratory syndrome coronavirus infection of mice transgenic for the human Angiotensin-converting enzyme 2 virus receptor.
• Skeletal muscle fiber type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression in fed and food-deprived rats.
• Synergistic inhibition of SARS-coronavirus replication by type I and type II IFN.
• The challenge of managing body weight in the modern world.
• Usefulness of fluorine-18 fluorodeoxyglucose positron emission tomography in patients with a residual structural abnormality after definitive treatment for squamous cell carcinoma of the head and neck.