Site-specific, covalent attachment of proteins to a solid surface.

Site-specific, covalent attachment of proteins to a solid surface. Research Abstract Details 

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Site-specific, covalent attachment of proteins to a solid surface. Abstract Text:

Benjamin P Duckworth,Juhua Xu,T Andrew Taton,Athena Guo,Mark D Distefano,

Immobilized and site-specifically labeled proteins are becoming invaluable tools in proteomics. Here, we describe a strategy to attach a desired protein to a solid surface in a covalent, site-specific manner. This approach employs an enzymatic posttranslational modification method to site-specifically label a target protein with an azide; an alternative substrate for protein farnesyl transferase containing an azide group was developed for this purpose. A bio-orthogonal Cu(I)-catalyzed cycloaddition reaction is then used to covalently attach the protein to agarose beads bearing an alkyne functional group. We demonstrate that both the azide incorporation and the capture steps can be performed on either a purified protein target or on a protein present within a complex mixture. This approach involves the use of a four-residue tag which is significantly smaller than most other tags reported to date and results in covalent immobilization of the target protein. Hence it should have significant applicability in protein science.

Site-specific, covalent attachment of proteins to a solid surface. Publishing Authors By Initials

BP Duckworth,J Xu,TA Taton,A Guo,MD Distefano,

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Site-specific, covalent attachment of proteins to a solid surface. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Bioconjugate chemistry

VOLUME: 17

Page Numbers: 967-74

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ISSN: 1043-1802

DAY: 3

MONTH: 12

YEAR: 2007

Site-specific, covalent attachment of proteins to a solid surface. Information

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LANGUAGE: eng

NlmUniqueID: 9010319

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AFFILIATION: Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.

Country: United States

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MEDLINETA: Bioconjug Chem

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