Site-specific collapse dynamics guide the formation of the cytochrome c' four-helix bundle.

Site-specific collapse dynamics guide the formation of the cytochrome c' four-helix bundle. Research Abstract Details 

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Site-specific collapse dynamics guide the formation of the cytochrome c' four-helix bundle. Abstract Text:

Tetsunari Kimura,Jennifer C Lee,Harry B Gray,Jay R Winkler,

The evolution of tryptophan-to-heme (W/heme) distance distributions extracted from analysis of fluorescence energy transfer kinetics during the refolding of Rhodopseudomonas palustris cytochrome c' reveals dramatic differences between two variants [W32 (Q1A/F32W/W72F) and W72 (Q1A)]. Both W32/heme and W72/heme distance distributions measured at the earliest time point attainable with a continuous-flow mixer (150 mus) confirm that the polypeptide ensemble is not uniformly collapsed and that native structure is not formed. Time-resolved fluorescence spectra indicate that W32 is sequestered from the aqueous solution during the first 700 mus of folding, whereas W72 remains exposed to solvent. The first moment of the W32/heme distance distribution evolves to its native value faster than that of W72, suggesting that the approach of W32 to the heme precedes that of W72.

Site-specific collapse dynamics guide the formation of the cytochrome c' four-helix bundle. Publishing Authors By Initials

T Kimura,JC Lee,HB Gray,JR Winkler,

For similar bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: bradyrhizobiaceae: rhodopseudomonas research abstracts see: bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: bradyrhizobiaceae: rhodopseudomonas research

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Site-specific collapse dynamics guide the formation of the cytochrome c' four-helix bundle. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 117-22

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 19

MONTH: 12

YEAR: 2006

Site-specific collapse dynamics guide the formation of the cytochrome c' four-helix bundle. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

Site-specific collapse dynamics guide the formation of the cytochrome c' four-helix bundle. Keywords Mesh Terms:

KEYWORDS: Rhodopseudomonas

MESH TERMS: enzymology

Chemical & Substance for Abstract: Site-specific collapse dynamics guide the formation of the cytochrome c' four-helix bundle. Information

Substance Name: Cytochromes c'

Registry Number: 0

Grant and Affiliation Information for Site-specific collapse dynamics guide the formation of the cytochrome c' four-helix bundle.

AFFILIATION: Beckman Institute, California Institute of Technology, Pasadena, CA 91125-7400, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM068461

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

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