Site-directed mutational analysis of DnaA protein, the initiator of chromosomal DNA replication in E. coli.

Site-directed mutational analysis of DnaA protein, the initiator of chromosomal DNA replication in E. coli. Research Abstract Details 

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Site-directed mutational analysis of DnaA protein, the initiator of chromosomal DNA replication in E. coli. Abstract Text:

T Mizushima,

DnaA protein, the initiator for chromosomal DNA replication in Escherichia coli, has various activities, such as oligomerization (DnaA-DnaA interaction), ATP-binding, ATPase activity and membrane-binding. Site-directed mutational analyses have revealed not only the amino acid residues that are essential for these activities but also the functions of these activities. Following is a summary of the functions and regulatory mechanisms of DnaA protein in the initiation of chromosomal DNA replication. ATP-bound DnaA protein, but not other forms of the protein binds to the origin of DNA replication and forms oligomers to open-up the duplex DNA. This oligomerization is mediated by a DnaA-DnaA interaction through the N-terminal region of the protein. After initiation of DNA replication, the ATPase activity of DnaA protein is stimulated and DnaA protein is inactivated to the ADP-bound form to suppress the re-initiation of DNA replication. DnaA protein binds to acidic phospholipids through an ionic interaction between basic amino acid residues of the protein and acidic residues of phospholipids. This interaction seems to be involved in the re-activation of DnaA protein (from the ADP-bound form to the ATP-bound form) to initiate DNA replication after the appropriate interval.

Site-directed mutational analysis of DnaA protein, the initiator of chromosomal DNA replication in E. coli. Publishing Authors By Initials

T Mizushima,

For similar investigative techniques: genetic techniques: genetic engineering: protein engineering: mutagenesis, site-directed research abstracts see: investigative techniques: genetic techniques: genetic engineering: protein engineering: mutagenesis, site-directed research

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Site-directed mutational analysis of DnaA protein, the initiator of chromosomal DNA replication in E. coli. Journal Published:

PUBLICATION TYPE: Review

Journal: Journal of biochemistry

VOLUME: 127

Page Numbers: 1-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 2000

Site-directed mutational analysis of DnaA protein, the initiator of chromosomal DNA replication in E. coli. Information

Number of References: 43

LANGUAGE: eng

NlmUniqueID: 376600

Site-directed mutational analysis of DnaA protein, the initiator of chromosomal DNA replication in E. coli. Keywords Mesh Terms:

KEYWORDS: Mutagenesis, Site-Directed

MESH TERMS: genetics

Chemical & Substance for Abstract: Site-directed mutational analysis of DnaA protein, the initiator of chromosomal DNA replication in E. coli. Information

Substance Name: DnaA protein, Bacteria

Registry Number: 0

Grant and Affiliation Information for Site-directed mutational analysis of DnaA protein, the initiator of chromosomal DNA replication in E. coli.

AFFILIATION: Faculty of Pharmaceutical Sciences, Okayama University, and PRESTO, Japan Science and Technology Corporation, Okayama 700-8530, Japan. mizushima@pheasant.pharm.okayama-u.ac.jp

Country: JAPAN

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MEDLINETA: J Biochem

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